{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:34:28Z","timestamp":1792290868039,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:00:00Z","timestamp":1673827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>p53 is an independent risk stratification marker in Barrett's oesophagus (BE), but no universally accepted definition exists for abnormal p53 staining. Herein, we assess p53 stains in two cohorts to: (1) define abnormal p53 staining in BE\u2010related dysplasia (BERD) and (2) assess the specificity and sensitivity of this cut\u2010point for the diagnosis of dysplasia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cohort 1 (<jats:italic>n<\/jats:italic>\u00a0=\u00a0313) included (1) dysplastic BE biopsies, (2) prior non\u2010dysplastic BE (NDBE) biopsies from the same patients and (3) NDBE biopsies from patients who never progressed to dysplasia. Cohort 2 (<jats:italic>n<\/jats:italic>\u00a0=\u00a0191) consisted of BE biopsies in which p53 staining aided in diagnosing dysplasia. Automated p53 staining quantification was performed on cohort 1. A semiquantitative p53 analysis, performed on both cohorts, included: (1) number of strongly positive glands, (2) strong glandular surface staining, (3) percentage of strongly positive glands and (4) null phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NDBE biopsies from cohort 1 patients who progressed to dysplasia were more likely to show p53 positivity than non\u2010progressors (16.9 versus 0.6%) (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0001). The optimal quantitative cut\u2010point for distinguishing dysplastic from never\u2010dysplasia biopsies was 10 strongly positive cells. By semiquantitative analysis, a single strongly p53\u2010positive gland distinguished dysplastic from never\u2010dysplasia BE (sensitivity 98.6%, specificity 99.4%). The semiquantitative and quantitative analyses correlated (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0001). In cohort 2, the sensitivity and specificity for BERD of \u2265\u20091 strongly positive p53 gland were 86.0 and 88.6%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A single strongly positive p53 gland is sensitive and specific for BERD. Automated p53 analysis may reduce subjectivity associated with the diagnosis of BERD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14848","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T13:45:11Z","timestamp":1669988711000},"page":"555-566","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Defining an abnormal p53 immunohistochemical stain in Barrett's oesophagus\u2010related dysplasia: a single\u2010positive crypt is a sensitive and specific marker of dysplasia"],"prefix":"10.1111","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1272-6119","authenticated-orcid":false,"given":"Kristen J","family":"Tomaszewski","sequence":"first","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5321-190X","authenticated-orcid":false,"given":"Azfar","family":"Neyaz","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenan","family":"Sauder","sequence":"additional","affiliation":[{"name":"Department of Pathology Newton\u2010Wellesley Hospital  Newton MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5224-7764","authenticated-orcid":false,"given":"Steffen","family":"Rickelt","sequence":"additional","affiliation":[{"name":"David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology  Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Lisa","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rory","family":"Crotty","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuti","family":"Shroff","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Odze","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Mattia","sequence":"additional","affiliation":[{"name":"Department of Pathology Newton\u2010Wellesley Hospital  Newton MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7837-1299","authenticated-orcid":false,"given":"Deepa T","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikram","family":"Deshpande","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Brigham and Women's Hospital  Boston MA 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