{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T15:25:29Z","timestamp":1785684329355,"version":"3.56.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T00:00:00Z","timestamp":1680480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012096","name":"Association Pour la Recherche et L'enseignement en Pathologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012096","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006364","name":"Institut National Du Cancer","doi-asserted-by":"publisher","award":["PRT\u2010K19\u2010143"],"award-info":[{"award-number":["PRT\u2010K19\u2010143"]}],"id":[{"id":"10.13039\/501100006364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim was to test the expression of PU.1 on different types of histiocytoses and to test the utility of PU.1 in confirming or excluding a histiocytic origin in tumour samples with suspicion of histiocytosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>We analysed 66 biopsies of nonmalignant histiocytoses represented by Langerhans\u2010cell histiocytosis (<jats:italic>n<\/jats:italic>\u2009=\u200913), Erdheim\u2013Chester disease (ECD) (<jats:italic>n<\/jats:italic>\u2009=\u200919), Rosai\u2013Dorfman disease (RDD) (<jats:italic>n<\/jats:italic>\u2009=\u200914), mixed ECD\u2010RDD (<jats:italic>n<\/jats:italic>\u2009=\u20093), ALK\u2010positive histiocytosis (<jats:italic>n<\/jats:italic>\u2009=\u20096), and juvenile xanthogranuloma (<jats:italic>n<\/jats:italic>\u2009=\u200911). All cases were positive for PU.1 in reactive and neoplastic histiocytes. In addition, 39 cases of tumours with high\u2010grade cytological atypia were referred to our center as suspicion of malignant histiocytosis\/histiocytic sarcoma and only 18 were confirmed. Indeed, more than half of these tumours (21\/39) were either undifferentiated malignant tumours with a stroma rich in histiocytes, diffuse large B\u2010cell lymphoma, or high\u2010grade dedifferentiated liposarcoma. PU.1 was useful to distinguish between the negativity of large atypical nuclei and the positivity of stromal reactive histiocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PU.1 is expressed by all types of histiocytosis. It distinguishes histiocytosis from histiocyte\u2010rich tumours with an easy interpretation due to its sharp nuclear staining. Its negativity in lesional\/tumour cells in histiocyte\u2010like lesions is useful to eliminate a histiocytosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14909","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T04:44:14Z","timestamp":1680583454000},"page":"320-325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["PU.1 is a useful nuclear marker to distinguish between histiocytosis and histiocyte\u2010rich tumours"],"prefix":"10.1111","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9541-0202","authenticated-orcid":false,"given":"Irena Antonia","family":"Ungureanu","sequence":"first","affiliation":[{"name":"Pathology Department Paris\u2010Saclay University, Versailles SQY University (UVSQ), EA4340\u2010BECCOH, Assistance Publique\u2013H\u00f4pitaux de Paris (APHP), Ambroise\u2010Par\u00e9 Hospital  Boulogne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fleur","family":"Cohen\u2010Aubart","sequence":"additional","affiliation":[{"name":"Internal Medicine Department 2, French National Referral Center for Rare Systemic Diseases and Histiocytoses Sorbonne University, APHP, Piti\u00e9\u2013Salp\u00eatri\u00e8re Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"H\u00e9ritier","sequence":"additional","affiliation":[{"name":"Pediatric Hematology Department, Referral Center for Histiocytoses Sorbonne University, APHP, Armand\u2010Trousseau Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Haroche","sequence":"additional","affiliation":[{"name":"Internal Medicine Department 2, French National Referral Center for Rare Systemic Diseases and Histiocytoses Sorbonne University, APHP, Piti\u00e9\u2013Salp\u00eatri\u00e8re Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Donadieu","sequence":"additional","affiliation":[{"name":"Pediatric Hematology Department, Referral Center for Histiocytoses, UVSQ, EA4340\u2010BECCOH APHP, Armand\u2010Trousseau Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Fran\u00e7ois","family":"Emile","sequence":"additional","affiliation":[{"name":"Pathology Department Paris\u2010Saclay University, Versailles SQY University (UVSQ), EA4340\u2010BECCOH, Assistance Publique\u2013H\u00f4pitaux de Paris (APHP), Ambroise\u2010Par\u00e9 Hospital  Boulogne France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14569"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-690636"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2015.06.001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21851"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-018-0704-1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-04-570937"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021013338"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202048881"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00228"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1219179"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31818866a7"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqac119"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1990.tb07791.x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000000880"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz125"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2022.05.012"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/030089161109700516"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001617"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428191003699845"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-023-03508-7"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14909","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T04:51:20Z","timestamp":1692334280000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.14909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,3]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1111\/his.14909"],"URL":"https:\/\/doi.org\/10.1111\/his.14909","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,3]]},"assertion":[{"value":"2022-10-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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