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In all, 180 SGSN tumours (formalin\u2010fixed, paraffin\u2010embedded samples, 141 specimens and 39 core needle biopsies) from the REFCORpath (French network for rare head and neck cancers) with previously identified fusion genes by fluorescent in situ hybridisation (FISH), RT\u2010PCR, or molecular immunohistochemistry were selected to test its specificity and sensitivity and validate its diagnostic use. Tested tumours encompassed 14 major tumours types, including secretory carcinoma, mucoepidermoid carcinoma, adenoid cystic carcinoma, salivary gland intraductal carcinoma, clear cell carcinoma, pleomorphic adenoma, adamantinoma\u2010like Ewing Sarcoma, <jats:italic>EWSR1::COLCA2<\/jats:italic> sinonasal sarcoma, <jats:italic>DEK::AFF2<\/jats:italic> sinonasal carcinoma, and biphenotypic sinonasal sarcoma. In\u2010frame fusion transcripts were detected in 97.8% of cases (176\/180). Gene fusion assay results correlated with conventional techniques (immunohistochemistry [IHC], FISH, and RT\u2010PCR) in 176\/180 tumours (97.8%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This targeted multiplexed <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>\u2010based <jats:styled-content style=\"fixed-case\">LD\u2010RT\u2010PCR<\/jats:styled-content> method is a robust, highly sensitive method for the detection of recurrent gene fusions from routine clinical <jats:styled-content style=\"fixed-case\">SGSN<\/jats:styled-content> tumours. It can be easily customized to cover new fusions. These results are promising for implementing an integrated <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content> system to rapidly\u00a0detect genetic aberrations, facilitating accurate, genomics\u2010based diagnoses, and accelerate time to precision therapies in <jats:styled-content style=\"fixed-case\">SGSN<\/jats:styled-content> tumours.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14971","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T23:25:18Z","timestamp":1687649118000},"page":"685-699","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detection of salivary gland and sinonasal fusions by a next\u2010generation sequencing based, ligation\u2010dependent, multiplex RT\u2010PCR assay"],"prefix":"10.1111","volume":"83","author":[{"given":"Marie\u2010Delphine","family":"Lanic","sequence":"first","affiliation":[{"name":"INSERM U1245, Cancer Center Henri Becquerel, Institute of Research and Innovation in Biomedicine (IRIB), University of Normandy, UNIROUEN  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Gu\u00e9rin","sequence":"additional","affiliation":[{"name":"Department of Pathology Centre Henri Becquerel  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Wassef","sequence":"additional","affiliation":[{"name":"Department of Pathology H\u00f4pital Lariboisi\u00e8re  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Durdilly","sequence":"additional","affiliation":[{"name":"Department of Pathology Centre Henri Becquerel  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinciane","family":"Rainville","sequence":"additional","affiliation":[{"name":"INSERM U1245, Cancer Center Henri Becquerel, Institute of Research and Innovation in Biomedicine (IRIB), University of Normandy, UNIROUEN  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Sater","sequence":"additional","affiliation":[{"name":"Department of Pathology Centre Henri Becquerel  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Jardin","sequence":"additional","affiliation":[{"name":"INSERM U1245, Cancer Center Henri Becquerel, Institute of Research and Innovation in Biomedicine (IRIB), University of Normandy, UNIROUEN  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Ruminy","sequence":"additional","affiliation":[{"name":"INSERM U1245, Cancer Center Henri Becquerel, Institute of Research and Innovation in Biomedicine (IRIB), University of Normandy, UNIROUEN  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Costes\u2010Martineau","sequence":"additional","affiliation":[{"name":"Department of Pathology CHU de Montpellier  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7448-9537","authenticated-orcid":false,"given":"Marick","family":"La\u00e9","sequence":"additional","affiliation":[{"name":"INSERM U1245, Cancer Center Henri Becquerel, Institute of Research and Innovation in Biomedicine (IRIB), University of Normandy, UNIROUEN  Rouen France"},{"name":"Department of Pathology Centre Henri Becquerel  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,22]]},"reference":[{"key":"e_1_2_14_2_1","volume-title":"Head and neck tumours","author":"WHO Classification of Tumours Editorial Board","year":"2022"},{"key":"e_1_2_14_3_1","unstructured":"Mitelman Database of Chromosome Aberrations and Gene Fusions in Cancer.MitelmanF JohanssonBandMertensFeds.https:\/\/mitelmandatabase.isb\u2010cgc.org;2022."},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3947"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.04.008"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000980"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22979"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23020"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.177"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2017.124"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-021-00536-2"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00980-x"},{"key":"e_1_2_14_13_1","first-page":"1338","article-title":"Ligation\u2010dependent RT\u2010PCR: a new specific and low\u2010cost technique to detect gene fusion in salivary gland tumors. 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