{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:18:34Z","timestamp":1785507514545,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Invasive lobular carcinoma (ILC) has distinct morphology and association with loss of E\u2010cadherin function. It has special clinical and imaging features, and its proper recognition is important. Following a recent proposal, we tested the value of the routine use\u00a0of\u00a0E\u2010cadherin immunohistochemistry (IHC) in recognizing ILC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Five pathologists with experience in breast pathology from four Hungarian institutions histotyped 1001 breast cancers from diagnostic core biopsies or excision specimens randomly assigned to haematoxylin and eosin (HE) diagnosis first, followed by E\u2010cadherin IHC; or to immediate HE and E\u2010cadherin\u2010based diagnosis. Of 524 cases with HE diagnosis, 73(14%) were deemed uncertain. E\u2010cadherin made the initial histological type change in 14\/524 cases (2.7%), including three with confident HE\u2010based type allocation. Use of E\u2010cadherin immunostaining was considered useful in 88\/477 cases (18%) with immediate dual assessment, and typing uncertainty went down to 5% (25\/477 cases), but was not zero. Collective assessment of 171 uncertain, difficult, nonclassical cases resulted in consensus diagnosis in most cases, but 15 cases were still doubtful as concerns their proper histological type.<jats:italic>CDH1<\/jats:italic>gene sequencing was attempted and successful in 13; pathogenic genetic alterations were identified in seven cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The routine use of E\u2010cadherin IHC decreases the uncertainty in typing and improves the typing accuracy at the cost of potentially redundant additional immunostains. Furthermore, this procedure does not exclude uncertainty due to E\u2010cadherin\u2010positive ILCs, which are occasionally difficult to confidently label as ILC, especially when the growth pattern is not classic.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15026","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:15:46Z","timestamp":1692778546000},"page":"810-821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of the routine use of E\u2010cadherin immunohistochemistry in the typing of breast carcinomas: results of a randomized diagnostic study"],"prefix":"10.1111","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1344-7744","authenticated-orcid":false,"given":"G\u00e1bor","family":"Cserni","sequence":"first","affiliation":[{"name":"Department of Pathology B\u00e1cs\u2010Kiskun County Teaching Hospital Kecskem\u00e9t Hungary"},{"name":"Department of Pathology, Albert Szent\u2010Gy\u00f6rgyi Medical Centre University of Szeged Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3995-9732","authenticated-orcid":false,"given":"Endre","family":"K\u00e1lm\u00e1n","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University of P\u00e9cs P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00f3ra","family":"Udvarhelyi","sequence":"additional","affiliation":[{"name":"Department of Surgical and Molecular Pathology Centre of Tumour Pathology, National Institute of Oncology Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eszter","family":"Papp","sequence":"additional","affiliation":[{"name":"Department of Surgical and Molecular Pathology Centre of Tumour Pathology, National Institute of Oncology Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-187X","authenticated-orcid":false,"given":"Isabel","family":"Grote","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8903-2345","authenticated-orcid":false,"given":"Stephan","family":"Bartels","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0891-7839","authenticated-orcid":false,"given":"Matthias","family":"Christgen","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Kreipe","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Hannover Medical School Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6498-5943","authenticated-orcid":false,"given":"Janina","family":"Kulka","sequence":"additional","affiliation":[{"name":"Department of Pathology, Forensic and Insurance Medicine Semmelweis University Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13153695"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-17-0603"},{"key":"e_1_2_12_4_1","unstructured":"Crizotinib in lobular breast diffuse gastric and triple negative lobular breast cancer or CDH1\u2010mutated solid tumours (ROLo). 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