{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:30:49Z","timestamp":1785771049295,"version":"3.56.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHR 1178\/3\u20101"],"award-info":[{"award-number":["SCHR 1178\/3\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Merkel cell carcinoma (MCC) is frequently caused by the Merkel cell polyomavirus (MCPyV). Characteristic for these virus\u2010positive (VP) MCC is MCPyV integration into the host genome and truncation of the viral oncogene Large T antigen (LT), with full\u2010length LT expression considered as incompatible with MCC growth. Genetic analysis of a VP\u2010MCC\/trichoblastoma combined tumour demonstrated that virus\u2010driven MCC can arise from an epithelial cell. Here we describe two further cases of VP\u2010MCC combined with an adnexal tumour, i.e. one trichoblastoma and one poroma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Whole\u2010genome sequencing of MCC\/trichoblastoma again provided evidence of a trichoblastoma\u2010derived MCC. Although an MCC\u2010typical LT\u2010truncating mutation was detected, we could not determine an integration site and we additionally detected a wildtype sequence encoding full\u2010length LT. Similarly, Sanger sequencing of the combined MCC\/poroma revealed coding sequences for both truncated and full\u2010length LT. Moreover, <jats:italic>in situ<\/jats:italic> RNA hybridization demonstrated expression of a late region mRNA encoding the viral capsid protein VP1 in both combined as well as in a few cases of pure MCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The data presented here suggest the presence of wildtype MCPyV genomes and VP1 transcription in a subset of MCC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15068","type":"journal-article","created":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T09:27:35Z","timestamp":1697189255000},"page":"356-368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Detection of wildtype Merkel cell polyomavirus genomic sequence and <scp>VP1<\/scp> transcription in a subset of Merkel cell 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