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Spatial transcriptomics describes a group of technologies that provide up to transcriptome\u2010wide expression profiling while preserving the spatial origin of the data, with many of these technologies able to provide these data using a single tissue section. Spatial transcriptomics allows expression profiling of highly specific areas within a tissue section potentially to subcellular resolution, and allows correlation of expression data with morphology, tissue type and location relative to other structures. While largely still research laboratory\u2010based, several spatial transcriptomics methods have now achieved compatibility with formalin\u2010fixed paraffin\u2010embedded tissue (FFPE), allowing their use in diagnostic tissue samples, and with further development potentially leading to their incorporation in routine anatomical pathology practice. 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