{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T18:19:54Z","timestamp":1792433994208,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004361","name":"Krebsliga Schweiz","doi-asserted-by":"publisher","award":["KFS\u20105228\u201002\u20102021"],"award-info":[{"award-number":["KFS\u20105228\u201002\u20102021"]}],"id":[{"id":"10.13039\/501100004361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Primary bone diffuse large B\u2010cell lymphoma (PB\u2010DLBCL) is not recognized as a separate entity by the current classification systems. Here we define and highlight its distinctive clinical presentation, morphology, phenotype, gene expression profile (GEP), and molecular genetics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected 27 respective cases and investigated their phenotype, performed gDNA panel sequencing covering 172 genes, and carried out fluorescence <jats:italic>in situ<\/jats:italic> hybridization to evaluate <jats:italic>MYC<\/jats:italic>, <jats:italic>BCL2,<\/jats:italic> and <jats:italic>BCL6<\/jats:italic> translocations. We attempted to genetically subclassify cases using the Two\u2010step classifier and performed GEP for cell\u2010of\u2010origin subtyping and <jats:italic>in silico<\/jats:italic> comparison to uncover up\u2010 and downregulated genes as opposed to other DLBCL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Most cases (<jats:italic>n<\/jats:italic>\u2009=\u200922) were germinal centre B\u2010cell\u2010like (GCB) by immunohistochemistry and all by GEP. Additionally, PB\u2010DLBCL had a mutational profile similar to follicular lymphoma and nodal GCB\u2010DLBCL, with the exception of more frequent <jats:italic>TP53<\/jats:italic> and <jats:italic>B2M<\/jats:italic> mutations. The GEP of PB\u2010DLBCL was unique, and the frequency of <jats:italic>BCL2<\/jats:italic> rearrangements was lower compared to nodal GCB\u2010DLBCL. The Two\u2010step classifier categorized eight of the cases as EZB, three as ST2, and one as MCD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study comprehensively characterizes PB\u2010DLBCL as a separate entity with distinct clinical and morpho\u2010molecular features. These insights may aid in developing tailored therapeutic strategies and shed light on its pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15096","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T21:03:16Z","timestamp":1700082196000},"page":"525-538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Primary bone diffuse large B\u2010cell lymphoma (PB\u2010DLBCL): a distinct extranodal lymphoma of germinal centre origin, with a common EZB\u2010like mutational profile and good prognosis"],"prefix":"10.1111","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5271-537X","authenticated-orcid":false,"given":"Vanesa\u2010Sindi","family":"Ivanova","sequence":"first","affiliation":[{"name":"Pathology Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Davies","sequence":"additional","affiliation":[{"name":"Leeds Institute for Data Analytics, University of Leeds  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0847-6156","authenticated-orcid":false,"given":"Thomas","family":"Menter","sequence":"additional","affiliation":[{"name":"Pathology Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian","family":"Wild","sequence":"additional","affiliation":[{"name":"Divison of Nuclear Medicine University Hospital Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute of Molecular Cancer Research, University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatime","family":"Krasniqi","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology University Hospital Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Stenner","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology University Hospital Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandros","family":"Papachristofilou","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology University Hospital Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Dirnhofer","sequence":"additional","affiliation":[{"name":"Pathology Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1100-3819","authenticated-orcid":false,"given":"Alexandar","family":"Tzankov","sequence":"additional","affiliation":[{"name":"Pathology Institute of Medical Genetics and Pathology, University Hospital Basel, University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"489","volume-title":"WHO classification of tumours of soft tissue and bone tumours","author":"Cleven AHG","year":"2020"},{"key":"e_1_2_12_3_1","volume-title":"WHO classification of tumours of soft tissue and bone","author":"Board WHO","year":"2013"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14124"},{"key":"e_1_2_12_5_1","article-title":"Primary bone lymphoma: a case series and review of literature","volume":"2020","author":"Bindal P","year":"2020","journal-title":"Case Rep. 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