{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:59:52Z","timestamp":1785578392909,"version":"3.56.0"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T00:00:00Z","timestamp":1714953600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Salivary gland neoplasms (SGN) exhibiting the\n                      <jats:italic>HMGA2<\/jats:italic>\n                      ::\n                      <jats:italic>WIF1<\/jats:italic>\n                      fusion are recognized by their resemblance to histology found in canalicular adenoma. Recently, ~20% of cases among 28\n                      <jats:italic>HMGA2<\/jats:italic>\n                      ::\n                      <jats:italic>WIF1<\/jats:italic>\n                      \u2010rearranged\u2010SGN showed malignancy and adverse outcomes (recurrence, distant metastasis, and disease\u2010specific mortality). Among them,\n                      <jats:italic>MDM2\/CDK4<\/jats:italic>\n                      amplifications were identified in one case. This outcome suggests that the\n                      <jats:italic>MDM2\/CDK4<\/jats:italic>\n                      amplifications could be useful to predict an aggressive course of carcinoma ex\u2010pleomorphic adenoma (CEPA).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      We investigated the correlation between\n                      <jats:italic>HMGA2<\/jats:italic>\n                      fusion and\n                      <jats:italic>MDM2<\/jats:italic>\n                      amplification in four salivary gland neoplasms, providing detailed clinicopathological features and outcomes. Cases were selected from different institutions. Histological examination, immunohistochemistry, fluorescence\n                      <jats:italic>in\u00a0situ<\/jats:italic>\n                      hybridization (FISH), RNA sequencing, and whole\u2010exome capture were performed. The cohort included four CEPA cases, all female, aged between 32 and 89\u2009years. Tumours arose from the parotid gland with an average size of 24.5\u2009mm. None exhibited recurrence or distant metastases during the 4\u20135\u2009months of follow\u2010up. Pathologically, all cases displayed a peculiar atypical nuclei with \u2018gear\u2010like appearance\u2019. Immunohistochemically, tumours exhibited a biphasic pattern with myoepithelial and ductal differentiation markers. All cases showed\n                      <jats:italic>HMGA2<\/jats:italic>\n                      overexpression and\n                      <jats:italic>MDM2<\/jats:italic>\n                      amplification by FISH and RNA sequencing. In a control cohort of\n                      <jats:italic>MDM2<\/jats:italic>\n                      nonamplified CEPA cases, not exhibiting the peculiar nuclear atypia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our findings suggest a strong correlation between\n                      <jats:italic>HMGA2<\/jats:italic>\n                      alteration\/\n                      <jats:italic>MDM2<\/jats:italic>\n                      amplification and a peculiar nuclear atypia, advocating for their evaluation in biphasic tumours to facilitate accurate diagnosis and tailored posttumour removal monitoring. Further studies are warranted to validate these observations and elucidate their prognostic implications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.15209","type":"journal-article","created":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T07:34:08Z","timestamp":1714980848000},"page":"338-346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Peculiar nuclear atypia associated with\n                    <i>MDM2<\/i>\n                    gene amplification in carcinoma ex\u2010pleomorphic adenoma harbouring an alteration of\n                    <i>HMGA2<\/i>"],"prefix":"10.1111","volume":"85","author":[{"given":"Ziyad","family":"Alsugair","sequence":"first","affiliation":[{"name":"Department of Pathology, Institut of Pathologie Multisite, Hospices Civils de Lyon University South Lyon Hospital  Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Fieux","sequence":"additional","affiliation":[{"name":"Department of Oto\u2010Rhino\u2010Laryngology and Head and Neck Surgery Hospices Civils de Lyon, Centre Hospitalier Lyon Sud  Pierre\u2010B\u00e9nite France"},{"name":"University Claude Bernard Lyon 1  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Descotes","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Biology Department Hospices Civils de Lyon, University South Lyon Hospital  Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Lopez","sequence":"additional","affiliation":[{"name":"University Claude Bernard Lyon 1  Lyon France"},{"name":"Biochemistry and Molecular Biology Department Hospices Civils de Lyon, University South Lyon Hospital  Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolle","family":"Cordonnier","sequence":"additional","affiliation":[{"name":"Department of Pathology University Amiens Hospital  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Russel","sequence":"additional","affiliation":[{"name":"Biopathology Department Centre Leon Berard  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Champagnac","sequence":"additional","affiliation":[{"name":"Biopathology Department Centre Leon Berard  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1118-950X","authenticated-orcid":false,"given":"Daniel","family":"Pissaloux","sequence":"additional","affiliation":[{"name":"Biopathology Department Centre Leon Berard  Lyon France"},{"name":"The Unit of Molecular Pathology, INSERM 1052, CNRS 5286 of Cancer Research Center of Lyon, and Team Genetics, Epigenetics and Biology of Sarcomas Universit\u00e9 Claude Bernard Lyon 1  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"C\u00e9ruse","sequence":"additional","affiliation":[{"name":"University Claude Bernard Lyon 1  Lyon France"},{"name":"Department of Oto\u2010Rhino\u2010Laryngology and Head and Neck Surgery Hospices Civils de Lyon, La Croix Rousse Hospital  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Philouze","sequence":"additional","affiliation":[{"name":"University Claude Bernard Lyon 1  Lyon France"},{"name":"Department of Oto\u2010Rhino\u2010Laryngology and Head and Neck Surgery Hospices Civils de Lyon, La Croix Rousse Hospital  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazim","family":"Benzerdjeb","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institut of Pathologie Multisite, Hospices Civils de Lyon University South Lyon Hospital  Pierre\u2010B\u00e9nite France"},{"name":"University Claude Bernard Lyon 1  Lyon France"},{"name":"EMR3738, CICLY  Pierre\u2010B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.08.017"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-013-0462-z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.15074"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20619"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64862-6"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjorl.2015.12.004"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.26137"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23009"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.216"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-013-0454-z"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20601"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.21873\/cdp.10250"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2264(2000)9999:9999<::AID-GCC1077>3.0.CO;2-B"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12127"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001783"}],"updated-by":[{"DOI":"10.1111\/his.15466","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000}}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.15209","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.15209","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.15209","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:42:05Z","timestamp":1785577325000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.15209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,6]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/his.15209"],"URL":"https:\/\/doi.org\/10.1111\/his.15209","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,6]]},"assertion":[{"value":"2023-12-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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