{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:26:50Z","timestamp":1788294410809,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T00:00:00Z","timestamp":1721779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Infections by the larval stage of the tape worms <jats:italic>Echinococcus multilocularis<\/jats:italic> and <jats:italic>Echinococcus granulosus s.l<\/jats:italic>. are potentially fatal zoonoses affecting humans as dead\u2010end hosts. Histopathological evaluation of hepatic echinococcosis is an integral part of patient management, including the distinction between alveolar (AE) and cystic echinococcosis (CE), which are associated with different disease courses and treatments. To improve histopathological assessment of <jats:italic>Echinococcus<\/jats:italic> lesions, we aimed to develop robust criteria to evaluate their viability and decay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Histomorphological criteria for determining parasitic viability based on the morphology of parasite structures and different stages of their decay were defined based on a clinically and molecularly defined cohort comprising 138 specimens from 112 patients (59 AE and 53\u2009CE); 618 AE lesions were assessed for histopathological viability comparing haematoxylin and eosin (H&amp;E) staining with mAbEm18 and mAbEm2G11 immunostaining. Moreover, parasite viability was systematically mapped in cross\u2010sections of five additional AE lesions. Protoscoleces in CE and AE displayed variable states of degeneration. Albendazole had no significant effect on the morphology of parasite structures. Viability assessment revealed high agreement between H&amp;E and mAbEm18, but not mAbEm2G11 staining, suggesting mAbEm18 staining as reliable for parasite viability assessment. H&amp;E and mAbEm18 staining displayed a central\u2013peripheral gradient of parasite viability and decay across parasitic lesions, with decayed cystic lesions located more towards the lesion centre while the most viable cystic lesions were located more peripherally.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Histopathological criteria corroborated by mAbEm18 staining provide a simple and reliable tool to assess the viability of AE lesions, knowledge of which is a valuable decision\u2010making tool for further treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15280","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T09:00:53Z","timestamp":1721811653000},"page":"857-867","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Histopathological assessment of the viability of hepatic alveolar echinococcosis"],"prefix":"10.1111","volume":"85","author":[{"given":"Selina","family":"Robers","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Pathology University of Zurich (UZH) and University Hospital Zurich (USZ)  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Reinehr","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University of Zurich (UZH) and University Hospital Zurich (USZ)  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lillemor","family":"Haibach","sequence":"additional","affiliation":[{"name":"Epidemiology, Biostatistics and Prevention Institute  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Furrer","sequence":"additional","affiliation":[{"name":"Epidemiology, Biostatistics and Prevention Institute  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annina","family":"Cincera","sequence":"additional","affiliation":[{"name":"Epidemiology, Biostatistics and Prevention Institute  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp Andreas","family":"Kronenberg","sequence":"additional","affiliation":[{"name":"Vetsuisse and Medical Faculty Institute of Parasitology, University of Zurich  Zurich Switzerland"},{"name":"Medical Micro\u2010 and Molecular Biology Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Zurich University of Applied Sciences (ZHAW)  W\u00e4denswil Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon Marc","family":"Eichenberger","sequence":"additional","affiliation":[{"name":"Vetsuisse and Medical Faculty Institute of Parasitology, University of Zurich  Zurich Switzerland"},{"name":"Medical Micro\u2010 and Molecular Biology Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Zurich University of Applied Sciences (ZHAW)  W\u00e4denswil Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Deplazes","sequence":"additional","affiliation":[{"name":"Vetsuisse and Medical Faculty Institute of Parasitology, University of Zurich  Zurich Switzerland"},{"name":"Department of Hepatology and Gastroenterology University of Zurich and University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ansgar","family":"Deibel","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology University of Zurich and University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beat","family":"M\u00fcllhaupt","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology University of Zurich and University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0073-3637","authenticated-orcid":false,"given":"Achim","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University of Zurich (UZH) and University Hospital Zurich (USZ)  Zurich Switzerland"},{"name":"Institute of Molecular Cancer Research, University of Zurich (UZH)  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000765"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2020024"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10101326"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.107-135.2004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.01.008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(20)30066-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1202.050499"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.11.001"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.029"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.11.002"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(95)80184-7"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-019-01922-6"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.953846"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010146"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15641-5"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001374"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001877"},{"key":"e_1_2_10_20_1","first-page":"148","article-title":"Hydatidosis (echinococcosis)","volume":"1","author":"Marty AM","year":"2000","journal-title":"Pathology of infectious diseases"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107161200"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000059278"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008921"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.41"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4161-4165.2002"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2760-2765.2002"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1162530"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fawpar.2019.e00055"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003964"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.112.109942"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2014072"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11020239"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000079300"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.09.006"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005636"},{"issue":"3","key":"e_1_2_10_36_1","first-page":"735","article-title":"Effect of chemotherapy on the larval mass and the long-term course of alveolar echinococcosis","volume":"19","author":"Ammann RW","year":"1994","journal-title":"Swiss Echinococcosis Study Group. Hepatology."},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.04.009"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01111-09"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hpb.2023.10.007"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.15280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T02:32:37Z","timestamp":1730946757000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.15280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,24]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1111\/his.15280"],"URL":"https:\/\/doi.org\/10.1111\/his.15280","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,24]]},"assertion":[{"value":"2024-03-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}