{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:09:07Z","timestamp":1790287747359,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The distinction between the benign subungual melanocytic lesions and an early lesion of subungual melanoma (SUM) remains a diagnostic challenge. We evaluated the routine diagnostic utility of array Comparative Genomic Hybridization (aCGH) to detect whole\u2010genome copy number variations (CNV) as well as targeted next\u2010generation sequencing (NGS) in SUM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>This retrospective study included 20 cases of <jats:italic>in situ<\/jats:italic> SUM and 11 cases of invasive SUM. Analysis by aCGH detected common oncogene amplifications in all but one case of invasive SUM (<jats:italic>n<\/jats:italic>\u2009=\u200910) and in all cases of <jats:italic>in situ<\/jats:italic> SUM with a melanocyte count (MC)\u2009&gt;45\/mm (<jats:italic>n<\/jats:italic>\u2009=\u20094 true positive) and the average number of CNV was 8.5. Thirteen remaining cases of <jats:italic>in situ<\/jats:italic> SUM gave false negative results (<jats:italic>n<\/jats:italic>\u2009=\u200913), owing to a lack of sufficient melanocytes to analyse (median MC of 35.35; range: 10.16\u201339.5). Molecular analysis failed in four cases (three <jats:italic>in situ<\/jats:italic> SUM and one invasive SUM) due to insufficient amounts of DNA. Across the whole cohort, the sensitivity of aCGH was 52%, but when adjusting the cutoff to MC\u2009&gt;45\/mm, the sensitivity was 93%. Targeted NGS was less informative than aCGH analyses in our series of SUM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>To distinguish malignant from benign lesions, especially <jats:italic>in situ<\/jats:italic> SUM versus atypical lentiginous melanocytic proliferations, aCGH analysis should be performed when the MC is above 45 melanocytes per linear millimetre. This pangenomic method can detect oncogene amplifications, as well as a number of CNV\u2009&gt;3, which strongly support the diagnosis of malignancy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15297","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T05:00:34Z","timestamp":1723611634000},"page":"214-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Subungual melanoma: molecular analysis of 31 cases from early stage to invasive melanoma"],"prefix":"10.1111","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6214-3416","authenticated-orcid":false,"given":"Christophe","family":"Perrin","sequence":"first","affiliation":[{"name":"Laboratoire Central d'Anatomie Pathologique Nice University Hospital  Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Coutts","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology Maidstone Hospital  Kent UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9reng\u00e8re","family":"Dadone\u2010Montaudi\u00e9","sequence":"additional","affiliation":[{"name":"Institute for Research on Cancer and Aging of Nice (IRCAN) CNRS UMR 7284\/INSERM U1081, University of Cote d'Azur (UCA) Nice University Hospital  Nice France"},{"name":"Department of Pathology and Molecular Oncology, Laboratoire d'Oncologie Mol\u00e9culaire Nice University Hospital  Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31826b74b8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7985-1943-5_17"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-199710000-00005"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000940"},{"key":"e_1_2_10_6_1","first-page":"529","article-title":"Heterogeneity of the site of origin of malignant melanoma in ungual areas: \u2018subungual\u2019 malignant melanoma may be a misnomer","volume":"126","author":"Saida T","year":"1992","journal-title":"Br. J. Dermatol."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(00)90137-3"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ocl.2019.03.003"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2018.08.033"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07368.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2008.00551.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31815c8578"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-0960.2001.00486.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.12634"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.14226"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001878"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000002143"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.14290"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000002584"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000002156"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/1066896916648379"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2018.03.030"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14121"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14279"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000688"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18988-3"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.27642"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15174417"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.06.2984"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000534955"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22083826"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.13034"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0499-y"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.05.010"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2006.06.007"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050092"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2003.49"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2021.12.293"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(96)90010-9"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.08.052"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.15297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T13:21:36Z","timestamp":1734700896000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.15297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,13]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/his.15297"],"URL":"https:\/\/doi.org\/10.1111\/his.15297","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,13]]},"assertion":[{"value":"2024-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}