{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:13:15Z","timestamp":1792811595583,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Threonine and tyrosine kinase (TTK) is up\u2010regulated in triple\u2010negative breast cancer (TNBC), yet its expression in patients undergoing neoadjuvant chemotherapy (NACT) remains unexplored. This investigation aims to assess TTK protein expression in treatment\u2010na\u00efve pre\u2010treatment cores and paired pre\u2010 and post\u2010NACT breast cancer (BC) cohorts, as well as its correlation with microcephaly 1 (MCPH1) protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Transcriptomic data were sourced from the Gene Expression Omnibus microarray database for mRNA expression analysis. TTK protein expression was evaluated using immunohistochemistry staining, employing receiver operating characteristic curve analysis to determine an optimal TTK expression cut\u2010off point. The association between TTK expression, clinicopathological parameters and survival outcomes was examined. Additionally, MCPH1 protein expression was assessed in a pilot study. Analysis revealed a significantly elevated TTK mRNA expression in BC tissue compared to normal breast tissue, with high TTK mRNA levels predicting reduced overall survival. Notably, TTK protein expression increased significantly post\u2010NACT in a paired cohort. Conversely, decreased TTK protein expression pre\u2010NACT was correlated with improved overall survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>High TTK and low MCPH1 protein expression was significantly correlated, highlighting TTK's potential as a biomarker for BC and a therapeutic target for MCPH1\u2010deficient cancer cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15399","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T06:50:02Z","timestamp":1736319002000},"page":"916-932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Threonine and tyrosine kinase (<scp>TTK<\/scp>) <scp>mRNA<\/scp> and protein expression in breast cancer; prognostic significance in the neoadjuvant setting"],"prefix":"10.1111","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3828-040X","authenticated-orcid":false,"given":"Abrar","family":"Ashi","sequence":"first","affiliation":[{"name":"Division of Molecular Medicine, Leeds Institute of Medical Research, St James's University Hospital University of Leeds  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6057-3973","authenticated-orcid":false,"given":"Aeshah A","family":"Awaji","sequence":"additional","affiliation":[{"name":"Division of Molecular Medicine, Leeds Institute of Medical Research, St James's University Hospital University of Leeds  Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5390-5688","authenticated-orcid":false,"given":"Jacquelyn","family":"Bond","sequence":"additional","affiliation":[{"name":"Division of Molecular Medicine, Leeds Institute of Medical Research, St James's University 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