{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:46:01Z","timestamp":1791665161478,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>RAF1\u2010rearranged spindle cell neoplasm represents a rare but distinctive entity in the category of kinase\u2010altered spindle cell neoplasms . We describe herein a cohort of six additional cases of RAF1\u2010rearranged spindle cell neoplasm to further broaden its clinicopathological and molecular spectrum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>The clinicopathological features, immunophenotypes and molecular profiles of six RAF1\u2010rearranged spindle cell neoplasms were assessed. A comprehensive review of the literature was performed. There were two males and four females with age at presentation ranging from 0 to 65\u2009years (median\u2009=\u200917\u2009years). Three tumours arose in the lower extremities, two in the head and neck region and one in the ileum. Morphological examination showed spindle cell tumours with variable cellularity and atypia, displaying solid growth (<jats:italic>n<\/jats:italic>\u2009=\u20094), infantile fibrosarcoma\u2010like (<jats:italic>n<\/jats:italic>\u2009=\u20091) and malignant peripheral nerve sheath tumour\u2010like (<jats:italic>n<\/jats:italic>\u2009=\u20091) patterns respectively. Using immunohistochemistry, three cases co\u2010expresssed CD34 and S100. Targeted RNA sequencing identified <jats:italic>PDZRN3::RAF1, FMR1::RAF1<\/jats:italic>, <jats:italic>PTPRG::RAF1<\/jats:italic>, <jats:italic>QKI::RAF1<\/jats:italic>, <jats:italic>SPPL2A::RAF1<\/jats:italic> and <jats:italic>ERC1::RAF1<\/jats:italic> fusion in one case each. <jats:italic>RAF1<\/jats:italic> rearrangements were subsequently substantiated by fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridisation. Follow\u2010up (14\u201348\u2009months) in four patients showed no signs of local recurrence or distant metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>RAF1\u2010rearranged spindle cell neoplasm encompasses a morphologically and molecularly diverse spectrum of mesenchymal tumours occurring in both children and adults. We describe an ileal lesion and two novel <jats:italic>SPPL2A::RAF1<\/jats:italic> and <jats:italic>ERC1::RAF1<\/jats:italic> fusions to further expand its clinicopathological and genetic spectrum.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.15445","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T12:09:12Z","timestamp":1743163752000},"page":"388-396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>RAF1<\/scp>\u2010rearranged spindle cell neoplasm: a clinicopathological and molecular genetic study of six cases with review of the literature"],"prefix":"10.1111","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7175-9856","authenticated-orcid":false,"given":"Peipei","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pathology Fudan University Shanghai Cancer Center, Fudan University  Shanghai China"},{"name":"Department of Oncology Shanghai 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