{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:11:15Z","timestamp":1786727475285,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T00:00:00Z","timestamp":1743724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T00:00:00Z","timestamp":1743724800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Demonstration of clonality is important in the diagnosis of a T\u00a0cell neoplasm. Allelic exclusion of T\u00a0cell receptor beta constant chains (\n                      <jats:italic>TRBC<\/jats:italic>\n                      ) ensures restricted TRBC1 or 2 expression in T\u00a0cells. Here, we extend the applicability of TRBC1 immunohistochemistry (IHC) in assessing T\u00a0cell clonality in formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue sections, with a particular focus on peripheral T\u00a0cell lymphoma and lymphoblastic lymphoma\/leukaemia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      TRBC1 and CD3 IHCs were performed on benign lymph nodes (BLN;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200921), mature T\u00a0cell lymphomas (TCL;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200934), thymic tissues (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200912) and T lymphoblastic lymphoma\/leukaemia (T\u2010ALL;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200921). TRBC1 usage in CD3+\u2009cells [TRBC1\/CD3(%)] was scored manually and computationally. Non\u2010restricted TRBC1 patterns in reactive T\u00a0cells, as measured by TRBC1\/CD3(%), are normally distributed (BLN average\u2009=\u200959.4%; thymocyte average\u2009=\u200958.5%). TRBC1 staining patterns, as measured by TRBC1\/CD3(%), distribute into three clusters, reflecting the monotypic populations (TRBC1+\u2009and TRBC1\u2013) at two ends and a third cluster with a non\u2010restricted pattern (due to increased reactive T\u00a0cells). Based on the distribution patterns, we suggest TRBC1\/CD3(%) \u2264\u20090.25 and\u2009\u2265\u20090.75 as a guide to establish monotypic\u00a0patterns in mature T\u00a0cells. Our T\u2010ALL cohort, selected for high blast burden, exhibits monotypic TRBC1 patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>TRBC1 IHC is a useful tool that can complement molecular TCR gene rearrangement studies in assessing clonality in mature and immature T\u00a0cell populations, and can be compatible with decalcified tissue.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.15454","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T10:08:46Z","timestamp":1743847726000},"page":"572-582","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Expanding the use of T\u00a0cell receptor beta constant 1 immunohistochemistry in formalin\u2010fixed paraffin\u2010embedded tissues in assessing T\u00a0cell clonality in mature and immature T\u00a0cell neoplasms"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7587-4035","authenticated-orcid":false,"given":"Winston Y","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5228-0628","authenticated-orcid":false,"given":"Young S","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8701-9504","authenticated-orcid":false,"given":"Jack","family":"Reid","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Davidson","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1836-6591","authenticated-orcid":false,"given":"Alexandra C","family":"Hristov","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5700-2141","authenticated-orcid":false,"given":"Anamarija M","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Michigan  Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorinda","family":"Soma","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3497-2513","authenticated-orcid":false,"given":"Joo Y","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pathology City of Hope National Medical Center  Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2020.09.024"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204761"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqy173"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4444"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174379"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-024-01002-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqab098"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21782"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-5323(02)00064-7"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2232"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2023.11.051"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-46607-w"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45854-3"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.181"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqab090"},{"key":"e_1_2_8_19_1","first-page":"365","article-title":"Application of a T\u00a0cell receptor antibody beta F1 for immunophenotypic analysis of malignant lymphomas","volume":"132","author":"Ng CS","year":"1988","journal-title":"Am. J. Pathol."},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21881"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2022.101552"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/lmac014"},{"key":"e_1_2_8_23_1","first-page":"1","article-title":"Treatment response and outcome of children with T\u00a0cell acute lymphoblastic leukemia expressing the gamma\u2010delta T\u00a0cell receptor","volume":"8","author":"Pui CH","year":"2019","journal-title":"Onco. Targets. 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