{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:52Z","timestamp":1787014312208,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Intraductal carcinoma of the prostate (IDC\u2010P) is a strong indicator of poor prognosis in prostate cancer (PCa). We utilized the Visium Spatial Gene Expression platform to characterize the gene expression profiles and copy number variations (CNVs) of IDC\u2010P.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      Manually annotated IDC\u2010P components were relatively enriched in a single transcriptomic cluster identified by principal component analysis, which exhibited elevated expression of\n                      <jats:italic>FOLH1<\/jats:italic>\n                      (PSMA),\n                      <jats:italic>PSCA<\/jats:italic>\n                      and\n                      <jats:italic>PLA2G2A<\/jats:italic>\n                      . Differential gene expression analysis between IDC\u2010P and non\u2010IDC\u2010P cancer tissues revealed up\u2010regulation of pathways related to chemotaxis and leukocyte migration, as well as gene sets associated with small cell lung carcinoma, suggesting a potential link to treatment\u2010related neuroendocrine prostate carcinoma. In contrast, non\u2010IDC\u2010P components showed increased expression of genes associated with extracellular matrix organization.\n                    <\/jats:p>\n                    <jats:p>InferCNV analysis identified distinct CNV patterns differentiating IDC\u2010P from non\u2010IDC\u2010P cancer components in four out of six cases. However, no common CNV alterations were shared across these cases, indicating molecular diversity among IDC\u2010P lesions. In the remaining cases, IDC\u2010P clustered with Gleason pattern 5 carcinoma, and no CNV alterations distinguishing IDC\u2010P from adjacent non\u2010IDC\u2010P components were identified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings suggest that IDC\u2010P represents a biologically distinct component from conventional acinar adenocarcinoma and may reflect spatial tumour progression through pre\u2010existing ductal structures. Our study also suggests that the molecular mechanisms underlying IDC\u2010P progression may differ between patients, while the limited sample size (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096) warrants cautious interpretation and further validation in larger cohorts.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.15551","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T09:27:13Z","timestamp":1758187633000},"page":"745-756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Spatial transcriptomics of intraductal carcinoma of the prostate"],"prefix":"10.1111","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9219-8357","authenticated-orcid":false,"given":"Taishi","family":"Takahara","sequence":"first","affiliation":[{"name":"Department of Surgical Pathology Aichi Medical University 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