{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:43:03Z","timestamp":1790678583102,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014008","name":"Ovarian Cancer Research Alliance","doi-asserted-by":"publisher","award":["MIG\u20102025\u20102\u20101954"],"award-info":[{"award-number":["MIG\u20102025\u20102\u20101954"]}],"id":[{"id":"10.13039\/100014008","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Metastatic pancreatic adenocarcinoma (PDAC), albeit uncommon, may involve the ovary, and distinction from primary ovarian mucinous tumours (OMT) poses a diagnostic challenge. Our aim was to develop an ancillary immunohistochemical (IHC) panel to aid in diagnosis and to validate the morphological features of metastatic PDAC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Six IHC markers (CDX2, CK17, MEP1A, MUC2, PAX8, SMAD4) selected based on a literature review were stained on tissue microarrays containing 256 PDAC, 102 mucinous ovarian carcinomas (MC) and 58 mucinous borderline ovarian tumours (MBOT). Detailed morphological features were reviewed in 16 ovarian metastases from PDAC, 25 MC, and 9 MBOT. We confirmed that tumours with a size less than 13\u2009cm, bilaterality, ovarian surface involvement, low\u2010power nodularity, infiltrative invasion, pseudomyxoma ovarii despite cystadenoma or borderline areas, and moderate nuclear atypia should raise suspicion for metastatic PDAC and prompt evaluation with the recommended IHC panel. A 5\u2010marker panel consisting of CK17, MEP1A, PAX8, SMAD4, and CDX2 had an overall accuracy of 91.8% (95% CI 88.8%\u201394.3%) using recursive partitioning, with the highest weight resting on CK17. CK17 was expressed in 80.9% of PDAC compared to 18.6% of MC and 1.7% of MBOT, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This is the first ancillary IHC panel to distinguish between PDAC and OMT with high accuracy. These results inform further studies on diagnostic workflows tailored to the complexity of metastatic presentations of tumours at the ovary.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70002","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T12:39:16Z","timestamp":1758285556000},"page":"402-413","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A 5\u2010marker immunohistochemical panel of\n                    <scp>CK17<\/scp>\n                    ,\n                    <scp>MEP1A<\/scp>\n                    ,\n                    <scp>PAX8<\/scp>\n                    ,\n                    <scp>SMAD4<\/scp>\n                    , and\n                    <scp>CDX2<\/scp>\n                    to distinguish ovarian mucinous carcinoma from metastatic pancreatic ductal adenocarcinoma"],"prefix":"10.1111","volume":"88","author":[{"given":"Astrid","family":"De Boeck","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Young","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9134-2118","authenticated-orcid":false,"given":"Nicola S","family":"Meagher","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Faculty of Medicine and Health University of NSW Sydney  Sydney New South Wales Australia"},{"name":"The Daffodil Centre, A Joint Venture With Cancer Council NSW The University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5681-2022","authenticated-orcid":false,"given":"Kylie L","family":"Gorringe","sequence":"additional","affiliation":[{"name":"Peter MacCallum Cancer Centre  Melbourne Victoria Australia"},{"name":"Sir Peter MacCallum Department of Medical Oncology The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2094-1222","authenticated-orcid":false,"given":"Cheng Han","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parham","family":"Minoo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6615-2037","authenticated-orcid":false,"given":"Martin","family":"K\u00f6bel","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-020-00714-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2009.24.1.114"},{"key":"e_1_2_10_4_1","unstructured":"Available from:https:\/\/gco.iarc.fr\/today\/en. Assessed 21.08.2025."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djv428"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-021-01079-2"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-019-0302-0"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.6407"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31820508d0"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000637"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000754"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-022-03338-z"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-22-1206"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.15128"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000274"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000967"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2005.02194.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2018-0289-RA"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3180690d2d"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318216c112"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.176"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0b013e318288b384"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0000000000000473"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-1228"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11862-x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/17588359211053412"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.6217"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.70002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T08:16:15Z","timestamp":1765786575000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.70002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,19]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/his.70002"],"URL":"https:\/\/doi.org\/10.1111\/his.70002","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,19]]},"assertion":[{"value":"2025-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}