{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:33:19Z","timestamp":1790605999211,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"\n Aims<\/jats:title>\n \n Testicular adult granulosa cell tumours (AGCTs) are rare and show several clinical\u2013pathological differences with their ovarian counterparts. In a limited number of prior studies,\n FOXL2<\/jats:italic>\n p.Cys134Trp, the hallmark molecular alteration of ovarian AGCT, appeared to be infrequent in testicular AGCTs. However, the number of cases analysed to date is relatively small.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and results<\/jats:title>\n \n Twenty testicular\n AGCTs<\/jats:styled-content>\n were analysed\n de novo<\/jats:italic>\n using two different next\u2010generation sequencing (\n NGS<\/jats:styled-content>\n ) panels that cover sex cord\u2010stromal tumour (\n SCST<\/jats:styled-content>\n )\u2013relevant genes, including\n \n FOXL2<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n CTNNB1<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n FH<\/jats:italic>\n <\/jats:styled-content>\n and\n \n DICER1<\/jats:italic>\n <\/jats:styled-content>\n . Among 12 tumours (12\/20; 60%) that were sequenced successfully, none harboured\n \n FOXL2<\/jats:italic>\n <\/jats:styled-content>\n mutations. Eight tumours (8\/12, 66.7%) showed a wild\u2010type (\n WT<\/jats:styled-content>\n ) status for all genes assessed with the panels. Three tumours harboured pathogenic or likely pathogenic\n \n CTNNB1<\/jats:italic>\n <\/jats:styled-content>\n alterations. One of these exhibited predominant spindle cell morphology, while the other two showed focal tubular architecture. Immunohistochemistry performed in one of these tumours with available material showed \u03b2\u2010catenin expression in ~70% of tumor cell nuclei. The remaining\n AGCTs<\/jats:styled-content>\n showed variants of uncertain significance (likely benign) in\n \n KIT<\/jats:italic>\n <\/jats:styled-content>\n and\n \n MED12<\/jats:italic>\n <\/jats:styled-content>\n . Considering the tumors asseseed in this study and those previously reported in the literature, only 2 of 29 neoplasms classified as testicular\n AGCTs<\/jats:styled-content>\n have shown a\n \n FOXL2<\/jats:italic>\n <\/jats:styled-content>\n p.\n Cys134Trp<\/jats:styled-content>\n mutation to date.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The present study confirms that SCSTs classified as AGCTs differ from their ovarian counterparts in that they largely lack\n FOXL2<\/jats:italic>\n mutations.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/his.70048","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T15:33:02Z","timestamp":1763652782000},"page":"831-842","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Adult granulosa cell tumours of the testis analogous to ovarian counterparts are exceptionally rare: analysis of a multicentric series and review of the literature"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7254-4195","authenticated-orcid":false,"given":"Costantino","family":"Ricci","sequence":"first","affiliation":[{"name":"Pathology Unit, DIAP\u2010Dipartimento Interaziendale di Anatomia Patologica di Bologna Maggiore Hospital\u2010AUSL Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0609-8817","authenticated-orcid":false,"given":"Dario","family":"de Biase","sequence":"additional","affiliation":[{"name":"Solid Tumor Molecular Pathology Laboratory IRCCS Azienda Ospedaliero\u2010Universitaria di Bologna Bologna Italy"},{"name":"Department of Pharmacy and Biotechnology (FaBiT) University of Bologna Bologna 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