{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:59:15Z","timestamp":1787061555724,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To understand whether the worldwide implementation of PD\u2010L1 testing in triple\u2010negative breast cancer (TNBC) can be achieved in routine clinical practice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>The multicentre retrospective observational VANESSA study consecutively and uniformly enrolled patients treated with systemic therapy for early or metastatic (e\/m)TNBC diagnosed between 2014 and 2017. PD\u2010L1 status was retrospectively assessed locally and centrally using the VENTANA PD\u2010L1 (SP142) Assay (PD\u2010L1 expression on tumour\u2010infiltrating immune cells covering \u22651% of the tumour area). The primary objective was to determine the prevalence of PD\u2010L1 positivity assessed locally on primary and\/or metastatic tumour tissue. Concordance between local and central testing was a secondary endpoint. PD\u2010L1\u2010positive prevalence was 38% in eTNBC (728\/1902) and 20% in mTNBC (30\/152) and was higher in submitted tissue size &gt;5 versus &lt;5\u2009mm diameter (eTNBC: 43% versus 16%; mTNBC: 24% versus 13%). Among 1967 samples tested both centrally and locally, concordance was 75% (Cohen's \u03ba coefficient 0.52, 95% CI 0.48\u20130.55) and was similar regardless of cohort (eTNBC versus mTNBC), sample collection method (biopsy versus resection) or sample origin (primary versus metastatic). PD\u2010L1\u2010positive prevalence was higher by central versus local assessment (eTNBC: 55% versus 39%; mTNBC: 26% versus 20%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this real\u2010world study, PD\u2010L1\u2010positive prevalence was lower than in prospective trials assessing PD\u2010L1 status centrally, lower in mTNBC than eTNBC, lower in smaller than larger tissue samples and lower by local than central assessment. These findings underline the importance of central PD\u2010L1 testing on sufficiently large samples to ensure optimal selection for therapies targeting PD\u2010(L)1 in mTNBC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70091","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T05:54:22Z","timestamp":1771826062000},"page":"1373-1384","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Real\u2010world prevalence of\n                    <scp>PD<\/scp>\n                    \u2010\n                    <scp>L1<\/scp>\n                    positivity in early\u2010stage\/metastatic triple\u2010negative breast cancer: primary results and pathology insights from the global retrospective observational\n                    <scp>VANESSA<\/scp>\n                    study"],"prefix":"10.1111","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8358-2597","authenticated-orcid":false,"given":"Corrado","family":"D'Arrigo","sequence":"first","affiliation":[{"name":"Poundbury Cancer Institute for Personalised Medicine  Dorchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2658-6439","authenticated-orcid":false,"given":"Sitki","family":"Tuzlali","sequence":"additional","affiliation":[{"name":"Tuzlali Pathology Laboratory  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1825-9876","authenticated-orcid":false,"given":"Romualdo","family":"Barroso\u2010Sousa","sequence":"additional","affiliation":[{"name":"Bras\u00edlia Hospital, Rede Am\u00e9ricas  Brasilia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9612-4224","authenticated-orcid":false,"given":"Nagi S","family":"El Saghir","sequence":"additional","affiliation":[{"name":"American University of Beirut Medical Center  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6421-7602","authenticated-orcid":false,"given":"Rebecca","family":"Dent","sequence":"additional","affiliation":[{"name":"National Cancer Center Singapore Duke\u2010NUS Medical School  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5834-782X","authenticated-orcid":false,"given":"Nata\u0161a","family":"Medi\u0107\u2010Miliji\u0107","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Oncology and Radiology of Serbia  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5743-0712","authenticated-orcid":false,"given":"Gyungyub","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Pathology, Asan Medical Center University of Ulsan College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3472-511X","authenticated-orcid":false,"given":"Shahin","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Pathology Aga Khan University  Nairobi Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0668-8339","authenticated-orcid":false,"given":"Tu Thai","family":"Anh","sequence":"additional","affiliation":[{"name":"Pathology Department Ho Chi Minh City Oncology Hospital  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3319-3983","authenticated-orcid":false,"given":"Alisan","family":"Zirtiloglu","sequence":"additional","affiliation":[{"name":"Global Product Development\/Medical Affairs Oncology F. 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