{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:14:49Z","timestamp":1783455289049,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJST2052"],"award-info":[{"award-number":["JPMJST2052"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Chronic active antibody\u2010mediated rejection (caABMR) is a major cause of late kidney allograft failure, yet reliable histological indicators of microvascular injury and therapeutic response remain insufficient. Complement C4d deposition reflects complement activation but does not fully capture endothelial remodelling, particularly in ABO\u2010incompatible transplantation. Plasmalemma vesicle\u2010associated protein\u20101 (PV\u20101), encoded by PLVAP and normally absent from glomerular endothelium, is upregulated during endothelial remodelling. We investigated whether glomerular PV\u20101 complements C4d in identifying active endothelial injury in caABMR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>A total of 429 allograft biopsies from 126 patients with caABMR and 345 surveillance biopsies from 94 stable recipients were analysed. Glomerular PV\u20101 and C4d immunofluorescence intensities were quantified and correlated with histopathological lesions, renal function, and donor\u2010specific antibodies, with stratification by ABO compatibility. Both PV\u20101 and C4d were associated with microvascular inflammation and transplant glomerulopathy. While C4d reflected cumulative complement activation and showed staining in ABO\u2010incompatible grafts, PV\u20101 specifically identified endothelial remodelling, particularly in lesions with double\u2010contour formation, and demonstrated dynamic reduction following B\u2010cell\u2010directed therapy. Low\u2010vacuum scanning electron microscopy correlated with the presence of de novo PV\u20101 expression in glomerular endothelial cells. In multivariable Cox models incorporating \u0394PV\u20101 and \u0394C4d with clinical covariates, \u0394PV\u20101 remained independently associated with death\u2010censored graft survival, whereas \u0394C4d did not.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Glomerular PV\u20101 functions as a dynamic marker of endothelial remodelling that complements the static C4d footprint of complement activation. Combined assessment of PV\u20101 and C4d captures distinct dimensions of microvascular pathology and may refine histopathological evaluation of injury and treatment response in caABMR.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70162","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:08:26Z","timestamp":1776769706000},"page":"372-382","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glomerular plasmalemma vesicle\u2010associated protein\u20101 as an endothelial remodelling marker complementing C4d in chronic active antibody\u2010mediated rejection"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8605-0695","authenticated-orcid":false,"given":"Yuto","family":"Igarashi","sequence":"first","affiliation":[{"name":"Department of Urology Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3640-3537","authenticated-orcid":false,"given":"Mayu","family":"Shimokawa","sequence":"additional","affiliation":[{"name":"Department of Anatomy Showa Medical University School of Medicine  Tokyo Japan"},{"name":"Internal Medicine (Nephrology) Showa Medical University Fujigaoka Hospital  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3502-3467","authenticated-orcid":false,"given":"Kunio","family":"Kawanishi","sequence":"additional","affiliation":[{"name":"Department of Anatomy Showa Medical University School of Medicine  Tokyo Japan"},{"name":"Department of Experimental Pathology, Institute of Medicine University of Tsukuba  Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3343-6674","authenticated-orcid":false,"given":"Toshihito","family":"Hirai","sequence":"additional","affiliation":[{"name":"Department of Urology Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7172-7049","authenticated-orcid":false,"given":"Hiroshi","family":"Seino","sequence":"additional","affiliation":[{"name":"Division of Pathology, Kidney Center Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8345-3627","authenticated-orcid":false,"given":"Tomokazu","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Urology Tokyo Women's Medical University  Tokyo Japan"},{"name":"Department of Organ Transplant Medicine Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7370-4728","authenticated-orcid":false,"given":"Hideki","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Urology Tokyo Women's Medical University  Tokyo Japan"},{"name":"Department of Organ Transplant Medicine Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1775-0943","authenticated-orcid":false,"given":"Toshio","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Urology Tokyo Women's Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15074"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009101065"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12111"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14625"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16474"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/678180"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2014.06.056"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00662.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tri.12936"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2022.04.016"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00234"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13203"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2007.01876.x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2022.10.010"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajt.2023.10.016"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2024.10.004"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.07820910"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2012.01544.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xkme.2021.06.009"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2019.07.015"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.70162","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.70162","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.70162","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T19:24:24Z","timestamp":1783452264000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.70162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,21]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/his.70162"],"URL":"https:\/\/doi.org\/10.1111\/his.70162","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,21]]},"assertion":[{"value":"2025-12-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}