{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:14:03Z","timestamp":1783455243398,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Plasmacytoid urothelial carcinoma (PUC) is an aggressive morphological subtype characterized by discohesive single\u2010cell infiltration and frequent loss of epithelial adhesion. Although p63 is widely regarded as a reliable urothelial lineage marker in conventional urothelial carcinoma, its expression may be reduced or absent in plasmacytoid tumours, creating an important diagnostic pitfall. This multi\u2010institutional study aimed to characterize the clinicopathological and immunohistochemical profile of PUC, with particular emphasis on p63 immunoreactivity and its relationship to adhesion\u2010related markers and other diagnostically relevant immunophenotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      A total of 93 cases initially diagnosed as PUC were retrospectively reviewed across multiple centres. After histopathological re\u2010evaluation, 70 confirmed cases were included. Immunohistochemical analysis was performed for p63, e\u2010cadherin, p120 catenin, TRPS1,\n                      <jats:italic>HER2\/Neu<\/jats:italic>\n                      . The cohort comprised 70 patients with a median age of 66.5\u2009years (IQR, 59.8\u201372.0) and a strong male predominance of 85.7% (60\/70). Most specimens were obtained from transurethral resections (64.3%, 45\/70) and radical cystectomy specimens (34.3%, 24\/70). The median tumour size was 3.0\u2009cm (IQR, 1.0\u20135.0), and the median proportion of plasmacytoid differentiation was 80% (IQR, 20\u2013100). Morphologically, tumours were classified as classic plasmacytoid in 65.7% (46\/70), pleomorphic in 22.9% (16\/70) and desmoplastic in 11.4% (8\/70). Concurrent variant histology was identified in 21.4% (15\/70), most commonly micropapillary (12.9%, 9\/70) and sarcomatoid (7.1%, 5\/70) components. Lymphovascular invasion was present in 51.4% (36\/70), and concomitant carcinoma in situ was detected in 35.7% (25\/70). Immunohistochemically, p63 expression was retained in only 10.0% of tumours (7\/70). HER2 membranous overexpression was observed in 58.6% (41\/70), while loss of E\u2010cadherin expression was highly prevalent (84.3%, 59\/70). Aberrant p120 catenin expression was common, with cytoplasmic localization in 72.9% (51\/70) and complete loss in 17.1% (12\/70). TRPS1 immunostaining was available in 54 tumours and was negative in 92.6% of evaluable cases (50\/54).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PUC demonstrates a distinctive immunophenotype characterized by frequent loss of p63 and disruption of the e\u2010cadherin\/p120 adhesion complex. Recognition of p63 attenuation, together with adhesion\u2010related markers and HER2 status, provides a useful diagnostic framework for distinguishing this aggressive subtype from conventional urothelial carcinoma and its mimickers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70172","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:46:42Z","timestamp":1778028402000},"page":"338-346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Marked attenuation of\n                    <scp>P63<\/scp>\n                    expression in plasmacytoid urothelial carcinoma with expanded immunohistochemical framework including E\u2010cadherin,\n                    <scp>P120<\/scp>\n                    ,\n                    <scp>HER2<\/scp>\n                    and\n                    <scp>TRPS1<\/scp>"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0537-3127","authenticated-orcid":false,"given":"Busra","family":"Yaprak Bayrak","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Medicine Kocaeli University  Kocaeli Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7747-0460","authenticated-orcid":false,"given":"Kemal","family":"Kosemehmetoglu","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Hacettepe University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4221-0845","authenticated-orcid":false,"given":"Busra","family":"Ozbek","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Kocaeli University  Kocaeli Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9875-8228","authenticated-orcid":false,"given":"Sercan","family":"Simsek","sequence":"additional","affiliation":[{"name":"Department of Pathology Kutahya Evliya Celebi Research and Training Hospital  Kutahya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0893-4444","authenticated-orcid":false,"given":"Murat","family":"Oktay","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sisli Hospitals Group  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5779-6797","authenticated-orcid":false,"given":"Ganime","family":"Coban","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Bezmialem Vak\u0131f University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0251-9597","authenticated-orcid":false,"given":"Dilara Irem","family":"Arslan Kahraman","sequence":"additional","affiliation":[{"name":"Department of Pathology Ankara Etlik City Hospital  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1973-1279","authenticated-orcid":false,"given":"Egemen","family":"Ak\u0131nc\u0131oglu","sequence":"additional","affiliation":[{"name":"Department of Pathology Gulhane Research and Training Hospital  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0156-0105","authenticated-orcid":false,"given":"Melek","family":"Buyukgok","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine Dokuz Eylul University  Izmir 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