{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:10:46Z","timestamp":1788858646881,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to develop a reproducible immunohistochemistry method for formalin\u2010fixed, paraffin\u2010embedded (FFPE) muscle sections and to assess its diagnostic utility for idiopathic inflammatory myopathies (IIM) in settings where snap\u2010frozen muscle biopsy is unavailable.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty cases were analysed: dermatomyositis (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095), inclusion body myositis (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20092), immune\u2010mediated necrotizing myopathy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095), overlap myositis (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091), unspecified myopathy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20093), mitochondrial myopathy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091), LGMD R2 dysferlin\u2010related muscular dystrophy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091), facioscapulohumeral muscular dystrophy (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091), and neurogenic muscle change (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091). Immunohistochemistry was performed on 3\u2010\u03bcm FFPE muscle sections using heat\u2010induced antigen retrieval with citrate\/EDTA buffer and overnight incubation at 4\u00b0C with antibodies against MHC\u2010I, MHC\u2010II, MAC, MxA, and p62. Frozen sections were analysed in parallel for comparison.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparable staining intensities and patterns for MHC\u2010II, MxA, and p62 were achieved in FFPE muscle sections. Although discrepancies between frozen and FFPE sections occurred in eight cases, each involving a single marker, no statistically significant differences in immunohistochemical results were observed for MHC\u2010II (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.4795), MxA (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.4795), and p62 (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.6171). Concordance between methods was high, reaching 90% for MHC\u2010II and MxA and 80% for p62. These isolated discrepancies may reflect variability in antigen expression associated with the segmental and patchy involvement characteristic of IIM. However, MHC\u2010I and MAC staining were unsuccessful in FFPE sections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Successful immunolabelling of MHC\u2010II, MxA, and p62 was achieved in FFPE muscle sections. These findings indicate that several diagnostically informative markers for IIM can be reliably assessed in FFPE muscle, providing a practical diagnostic option in resource\u2010constrained settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70191","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T07:41:09Z","timestamp":1781077269000},"page":"699-709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic utility of inflammatory markers in formalin\u2010fixed and paraffin\u2010embedded muscle biopsies for idiopathic inflammatory myopathies"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3987-1797","authenticated-orcid":false,"given":"Chinnawut","family":"Suriyonplengsaeng","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Science Mahidol University  Bangkok Thailand"},{"name":"Clinical Anatomy Research and Education Laboratory (CARE Lab), Faculty of Science Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6744-6424","authenticated-orcid":false,"given":"Jariya","family":"Waisayarat","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine Ramathibodi Hospital Mahidol University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211468"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2018.2598"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0000000000000740"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/imj.15358"},{"key":"e_1_2_12_6_1","first-page":"178","article-title":"The classification of myositis: setting the stage for a universal terminology","volume":"43","author":"Giannini M","year":"2025","journal-title":"Clin. 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