{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:10:50Z","timestamp":1788858650349,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["KWF 150591"],"award-info":[{"award-number":["KWF 150591"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008359","name":"Maag Lever Darm Stichting","doi-asserted-by":"publisher","award":["MLDS WO 21\u201025"],"award-info":[{"award-number":["MLDS WO 21\u201025"]}],"id":[{"id":"10.13039\/501100008359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Evidence supporting the use of p53 immunohistochemistry (p53\u2010IHC) in Barrett's oesophagus (BE) is largely based on selected series, limiting generalizability. This study evaluates p53\u2010IHC in a nationwide, real\u2010world BE\u2010cohort within a universal healthcare system, assessing concordance with referring hospitals, interobserver agreement (IOA) and its impact on routine diagnostic decisions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Revision cases submitted to the Dutch Esophageal Pathology Panel (DEPP) were assessed by trained and experienced pathologists for haematoxylin and eosin (H&amp;E)\u2010based classification (Vienna classification), p53\u2010IHC patterns (wild\u2010type, overexpression, double clone, null mutation and equivocal) and combined H&amp;E\/p53\u2010IHC review. Concordance between referring hospitals and the expert panel (Cohen's kappa) and IOA among panel pathologists (Fleiss' kappa) were assessed, along with the impact of p53\u2010IHC on diagnostic reclassification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 1,146 consecutive patient consultation cases (1,704 biopsy levels) were included, with a median of 12 DEPP pathologist assessments per biopsy. Overall concordance of p53 assessment between referring hospitals and the expert panel was moderate\u2010to\u2010substantial (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.61, 95% CI 0.58\u20130.64), with 24.2% of p53\u2010IHC assessments reclassified. IOA among 13 pathologists assessing 316 endoscopic biopsies was substantial (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.65, 95% CI 0.61\u20130.69). Ancillary p53\u2010IHC influenced pathologists' decisions across biopsy levels. For non\u2010dysplastic (NDBE) biopsies, aberrant p53\u2010IHC prompted reclassification to indefinite for dysplasia (IND, 9.2%) or low\u2010grade dysplasia (LGD, 4.8%). For IND biopsies, p53\u2010IHC shifted diagnoses toward NDBE (17.0%) or LGD (37.1%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion\/discussion<\/jats:title>\n                    <jats:p>p53\u2010IHC interpretation is reliable, reproducible and clinically meaningful in BE diagnostics when standardized criteria are applied. These findings support broader use of p53\u2010IHC in routine practice and incorporation of assessment criteria into guidelines.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70193","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T11:26:27Z","timestamp":1781868387000},"page":"614-623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic impact and reproducibility of p53 immunohistochemistry in Barrett's oesophagus: results of the Dutch Esophageal Pathology Panel (\n                    <scp>DEPP<\/scp>\n                    )"],"prefix":"10.1111","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1366-0864","authenticated-orcid":false,"given":"Ylva A.","family":"Weeda","sequence":"first","affiliation":[{"name":"Department of Pathology Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"},{"name":"Cancer Center Amsterdam, Biomarkers  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6756-9587","authenticated-orcid":false,"given":"Sarah M.","family":"Issa","sequence":"additional","affiliation":[{"name":"Department of Pathology Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5752-0806","authenticated-orcid":false,"given":"Hans","family":"Halfwerk","sequence":"additional","affiliation":[{"name":"Department of Pathology Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4744-8315","authenticated-orcid":false,"given":"Michel","family":"Botros","sequence":"additional","affiliation":[{"name":"Department of Pathology Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"},{"name":"Department of Biomedical Engineering and Physics Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8844-1326","authenticated-orcid":false,"given":"Onno J.","family":"de Boer","sequence":"additional","affiliation":[{"name":"Department of Pathology Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1341-8994","authenticated-orcid":false,"given":"Lodewijk A.","family":"Brosens","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Utrecht, Utrecht University  Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8611-840X","authenticated-orcid":false,"given":"Michail","family":"Doukas","sequence":"additional","affiliation":[{"name":"Department of Pathology Erasmus University Medical Center  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3006-5978","authenticated-orcid":false,"given":"Gursah","family":"Kats\u2010Ugurlu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University Medical Center Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8888-0588","authenticated-orcid":false,"given":"Fiebo","family":"ten Kate","sequence":"additional","affiliation":[{"name":"Department of Pathology Isala Klinieken  Zwolle the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaap","family":"van der Laan","sequence":"additional","affiliation":[{"name":"Department of Pathology HAGA Hospital  The Hague the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ineke","family":"van Lijnschoten","sequence":"additional","affiliation":[{"name":"Catharina Hospital, PAMM  Eindhoven the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariadne H. A. G.","family":"Ooms","sequence":"additional","affiliation":[{"name":"Department of Pathology Erasmus University Medical Center  Rotterdam the Netherlands"},{"name":"Pathan BV  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6168-2381","authenticated-orcid":false,"given":"Lindsey","family":"Oudijk","sequence":"additional","affiliation":[{"name":"Department of Pathology Erasmus University Medical Center  Rotterdam the Netherlands"},{"name":"Department of Pathology Maasstad Hospital  Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7531-9599","authenticated-orcid":false,"given":"Rachel S.","family":"van der Post","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2977-5737","authenticated-orcid":false,"given":"Mihaela G.","family":"Raicu","sequence":"additional","affiliation":[{"name":"Department of Pathology St. Antonius Hospital  Nieuwegein the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4861-3415","authenticated-orcid":false,"given":"Bert","family":"Timmer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Medical Biology University Medical Center Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5825-3236","authenticated-orcid":false,"given":"Myrtle J.","family":"van der Wel","sequence":"additional","affiliation":[{"name":"Department of Pathology Amsterdam University Medical Center at the University of Amsterdam  Amsterdam the Netherlands"},{"name":"Department of 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