{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:40:07Z","timestamp":1792672807800,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Breast phyllodes tumours (PTs) are rare biphasic fibroepithelial neoplasms, classified into benign, borderline and malignant categories. Stromal mitotic activity is a key criterion in grading PT, but the methodology for mitotic assessment has not been clearly articulated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>We discuss the approach to determining stromal mitotic activity in PTs. Whether the most mitotically active area (hotspot) or an average count across several tumour regions should be adopted is reviewed. We also outline the operational protocol for mitotic hotspot evaluation, accounting for different microscope models with varying field diameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A standardized method of determining stromal mitoses in PTs is recommended in order to improve interobserver reproducibility and accuracy in grading PTs. The application of digital tools in stromal mitotic counting is also addressed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We advocate using the hotspot method of obtaining the highest stromal mitotic count, together with other histological parameters, to classify PTs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.70293","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:12:03Z","timestamp":1791461523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mitoses counting in phyllodes tumours: rationale for a standardized hotspot\u2010based approach"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0613-8054","authenticated-orcid":false,"given":"Edward","family":"Hadi","sequence":"first","affiliation":[{"name":"Diagnos Laboratorium Utama  Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8078-1630","authenticated-orcid":false,"given":"Mihir","family":"Gudi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine KK Women's and Children's Hospital  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0995-1721","authenticated-orcid":false,"given":"Xiaoxian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine East Carolina University  Greenville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9912-0245","authenticated-orcid":false,"given":"Sandra","family":"O'Toole","sequence":"additional","affiliation":[{"name":"Royal Prince Alfred Hospital and NSW Health Pathology, School of Medical Sciences, University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3051-2280","authenticated-orcid":false,"given":"Ales","family":"Ryska","sequence":"additional","affiliation":[{"name":"The Fingerland Department of Pathology Charles University Medical Faculty and University Hospital  Hradec Kralove Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9405-9665","authenticated-orcid":false,"given":"Gary","family":"Tse","sequence":"additional","affiliation":[{"name":"Department of Anatomical and Cellular Pathology The Chinese University of Hong Kong  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4067-2760","authenticated-orcid":false,"given":"Sunil","family":"Lakhani","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Queensland Centre for Clinical Research, Pathology Queensland and Sullivan Nicolaides Pathology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3608-7828","authenticated-orcid":false,"given":"Stuart","family":"Schnitt","sequence":"additional","affiliation":[{"name":"Department of Pathology Beth Israel Deaconess Medical Center and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5292-8474","authenticated-orcid":false,"given":"Ian","family":"Ellis","sequence":"additional","affiliation":[{"name":"Department of Histopathology Nottingham University Hospitals  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5009-5525","authenticated-orcid":false,"given":"Emad","family":"Rakha","sequence":"additional","affiliation":[{"name":"Academic Unit for Translational Medical Sciences School of Medicine, University of Nottingham  Nottingham UK"},{"name":"Department of Pathology Hamad Medical Corporation  Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1405-2878","authenticated-orcid":false,"given":"Puay Hoon","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine KK Women's and Children's Hospital  Singapore Singapore"},{"name":"Luma Medical Centre  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Breast tumours. WHO classification of tumours series","author":"WHO Classification of Tumours Editorial Board","year":"2026"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12876"},{"key":"e_1_2_8_4_1","volume-title":"WHO classification of tumours of the breast","author":"WHO Classification of Tumours Editorial Board","year":"2019"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2022.106969"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-007-9439-z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2011-200368"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-013-2684-x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2014-202215"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1327"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-018-0032-8"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/esmoopen-2020-000843"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqac055"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000002264"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2021-207742"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0533-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.5858\/134.4.e31"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2017.7042"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00981-w"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.70293","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.70293","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.70293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:37:35Z","timestamp":1792669055000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.70293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,8]]},"references-count":18,"alternative-id":["10.1111\/his.70293"],"URL":"https:\/\/doi.org\/10.1111\/his.70293","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,8]]},"assertion":[{"value":"2026-07-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"his.70293"}}