{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:47:15Z","timestamp":1771656435880,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T00:00:00Z","timestamp":1353542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Objectives<\/jats:title>The aim of the study was to qualitatively and semiquantitatively characterize the expression of the principal HIV<\/jats:styled-content> co\u2010receptors chemokine (C<\/jats:styled-content>\u2010C<\/jats:styled-content> motif) receptor 5 (CCR5<\/jats:styled-content>) and chemokine (C<\/jats:styled-content>\u2010X<\/jats:styled-content>\u2010C<\/jats:styled-content> motif) receptor 4 (CXCR4<\/jats:styled-content>) on susceptible CD4 T<\/jats:styled-content>\u2010helper cell, monocyte\/macrophage and L<\/jats:styled-content>angerhans dendritic cell populations within the cervical epithelia of asymptomatic women attending a genitourinary medicine clinic.<\/jats:p><\/jats:sec>Methods<\/jats:title>Of 77 asymptomatic women recruited, 35 were excluded: 21 because they were found to have bacterial vaginosis, eight because they were found to have candida and six for other reasons. Cervical cytobrush samples from 11 women with C<\/jats:styled-content>hlamydia trachomatis<\/jats:italic> infection and 31 women without any detectable genital infection were stained with fluorescently labelled antibodies specific for cell surface CCR5<\/jats:styled-content>, CXCR4<\/jats:styled-content>, CD4<\/jats:styled-content>, CD3<\/jats:styled-content>, CD1a<\/jats:styled-content> and CD19<\/jats:styled-content> expression, then analysed by flow cytometry.<\/jats:p><\/jats:sec>Results<\/jats:title>CD4<\/jats:styled-content>\/CD3 T<\/jats:styled-content>\u2010helper cells (84%), CD1a L<\/jats:styled-content>angerhans dendritic cells (75%) and CD4<\/jats:styled-content>\/CD14<\/jats:styled-content> monocytes\/macrophages (59%) were detected in the samples. CCR5<\/jats:styled-content> and CXCR4 HIV<\/jats:styled-content> co\u2010receptor expression was observed on 46\u201386% of the above subsets. CD1a<\/jats:styled-content> cells exhibited significantly higher CCR5<\/jats:styled-content> and CXCR4<\/jats:styled-content> positivity and median fluorescence than CD4<\/jats:styled-content> cells and higher CXCR4<\/jats:styled-content> positivity and median fluorescence than CD14<\/jats:styled-content> cells (P<\/jats:styled-content><\/jats:italic>\u2009<\u20090.05 or less). Increased detection of CCR5<\/jats:styled-content> over CXCR4<\/jats:styled-content> was seen in CD14<\/jats:styled-content> cells (P<\/jats:styled-content><\/jats:italic>\u2009<\u20090.05). No significant differences in CCR5<\/jats:styled-content> or CXCR4<\/jats:styled-content> expression were found in samples from asymptomatic women with or without chlamydial infection.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Co\u2010receptor expression confirms the potential for CD1a<\/jats:styled-content> Langerhans cells, monocytes\/macrophages and T<\/jats:styled-content>\u2010helper cells in the cervix as primary targets for HIV<\/jats:styled-content> infection. Previously observed selective transmission of CCR5<\/jats:styled-content>\u2010tropic isolates cannot be accounted for by a lack of CXCR4<\/jats:styled-content>\u2010expressing CD4<\/jats:styled-content> cervical immune cells. We were unable to identify any specific impact of chlamydial infection on co\u2010receptor expression in this study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hiv.12002","type":"journal-article","created":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T06:44:06Z","timestamp":1353566646000},"page":"108-114","source":"Crossref","is-referenced-by-count":6,"title":["HIV<\/scp>\u20101 co\u2010receptor expression and epithelial immune cells of the cervix in asymptomatic women attending a genitourinary medicine clinic"],"prefix":"10.1111","volume":"14","author":[{"given":"CP","family":"McClure","sequence":"first","affiliation":[{"name":"Virus Research Group Biomedical Research Unit Molecular Medical Sciences University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CA","family":"Bowman","sequence":"additional","affiliation":[{"name":"Sheffield Teaching Hospitals Foundation Trust Royal Hallamshire Hospital Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Geary","sequence":"additional","affiliation":[{"name":"Department of Infection and Immunity University of Sheffield Medical School Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Ryan","sequence":"additional","affiliation":[{"name":"Sheffield Teaching Hospitals Foundation Trust Royal Hallamshire Hospital Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JK","family":"Ball","sequence":"additional","affiliation":[{"name":"Virus Research Group Biomedical Research Unit Molecular Medical Sciences University of Nottingham Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Eley","sequence":"additional","affiliation":[{"name":"Department of Infection and Immunity University of Sheffield Medical School Sheffield UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,22]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"UNAIDS.The global AIDS epidemic \u2013 key facts.2012; 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