{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T15:40:03Z","timestamp":1716824403331},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,6,9]],"date-time":"2014-06-09T00:00:00Z","timestamp":1402272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Direcci\u00ef\u00bf\u00bdn Gerencia del Servicio Andaluz de Salud. Consejer\u00ef\u00bf\u00bda de Salud. Junta de Andaluc\u00ef\u00bf\u00bda","award":["SAS 111237"],"award-info":[{"award-number":["SAS 111237"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the changes on the<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>protease gene in plasma and peripheral blood mononuclear cell (<jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>) compartments during viral replication episodes in patients on boosted\u2010darunavir monotherapy (<jats:styled-content style=\"fixed-case\">mtDRV<\/jats:styled-content>\/rtv).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective study was carried out in which adult<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101\u2010infected patients who started<jats:styled-content style=\"fixed-case\">mtDRV<\/jats:styled-content>\/rtv after viral suppression for \u22656 months with no major darunavir\u2010related resistance mutations were enrolled. Patients with two consecutive plasma<jats:styled-content style=\"fixed-case\">HIV RNA<\/jats:styled-content>measurements &gt;\u2009200<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u20101<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>copies\/mL were considered as having virological failure (<jats:styled-content style=\"fixed-case\">VF<\/jats:styled-content>), while patients with two consecutive plasma<jats:styled-content style=\"fixed-case\">HIV RNA<\/jats:styled-content>measurements &gt;\u200950\u2009copies\/mL without meeting the<jats:styled-content style=\"fixed-case\">VF<\/jats:styled-content>criteria were considered to have virological rebound (<jats:styled-content style=\"fixed-case\">VR<\/jats:styled-content>).<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>protease genotypic profiles from plasma and<jats:styled-content style=\"fixed-case\">PBMCs<\/jats:styled-content>were performed at baseline and at<jats:styled-content style=\"fixed-case\">VF<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">VR<\/jats:styled-content>episodes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred and fifty patients were included in the study, with overall<jats:styled-content style=\"fixed-case\">VF<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">VR<\/jats:styled-content>rates of 14% (<jats:italic>n<\/jats:italic>\u2009=\u200921) and 14.7% (<jats:italic>n<\/jats:italic>\u2009=\u200922), respectively. No major darunavir resistance mutations were observed in the plasma or<jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>samples. Circulating and cell\u2010associated viruses showed a wild\u2010type protease gene sequence in 54% and 23% of patients, respectively while the remainder patients only harboured minor protease inhibitor\u2010associated mutations. Full concordance between plasma<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">PBMC DNA<\/jats:styled-content>protease genotypes was found in 23% of the sequences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>No darunavir\u2010related mutations were found in patients with<jats:styled-content style=\"fixed-case\">VF<\/jats:styled-content>or<jats:styled-content style=\"fixed-case\">VR<\/jats:styled-content>, either in plasma or in<jats:styled-content style=\"fixed-case\">PBMCs<\/jats:styled-content>; thus, simplification to<jats:styled-content style=\"fixed-case\">mtDRV<\/jats:styled-content>\/rtv does not comprise future antiretroviral treatment options.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hiv.12170","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T01:13:02Z","timestamp":1402362782000},"page":"57-61","source":"Crossref","is-referenced-by-count":5,"title":["Low concordance and resistance mutation emergence in the<scp>HIV<\/scp>protease gene among circulating and cell\u2010associated viruses at viral replication episodes during darunavir\/ritonavir monotherapy"],"prefix":"10.1111","volume":"16","author":[{"given":"A","family":"Torres\u2010Cornejo","sequence":"first","affiliation":[{"name":"Service of Infectious Diseases, Microbiology and Preventive Medicine University Hospital Virgen del Roc\u00edo Biomedicine Institute of Sevilla (IBiS) University Hospital Virgen del Roc\u00edo\/CSIC\/University of Sevilla Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"OJ","family":"Benmarzouk\u2010Hidalgo","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Microbiology and Preventive Medicine University Hospital Virgen del Roc\u00edo Biomedicine Institute of Sevilla (IBiS) University Hospital Virgen del Roc\u00edo\/CSIC\/University of Sevilla Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Gutierrez\u2010Valencia","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Microbiology and Preventive Medicine University Hospital Virgen del Roc\u00edo Biomedicine Institute of Sevilla (IBiS) University Hospital Virgen del Roc\u00edo\/CSIC\/University of Sevilla Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Ruiz\u2010Valderas","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Microbiology and Preventive Medicine University Hospital Virgen del Roc\u00edo Biomedicine Institute of Sevilla (IBiS) University Hospital Virgen del Roc\u00edo\/CSIC\/University of Sevilla Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Viciana","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Microbiology and Preventive Medicine University Hospital Virgen del Roc\u00edo Biomedicine Institute of Sevilla (IBiS) University Hospital Virgen del Roc\u00edo\/CSIC\/University of Sevilla Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LF","family":"L\u00f3pez\u2010Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Microbiology and Preventive Medicine University Hospital Virgen del Roc\u00edo Biomedicine Institute of Sevilla (IBiS) University Hospital Virgen del Roc\u00edo\/CSIC\/University of Sevilla Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-6405-10-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir518"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32834ef5d9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00126334-200110010-00001"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.20.10948"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki038"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200405210-00008"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP1710"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks052"},{"key":"e_1_2_6_11_1","first-page":"138","article-title":"Update of the drug resistance mutations in HIV\u20101: December 2009","volume":"17","author":"Johnson VA","year":"2009","journal-title":"Top HIV Med"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000000060"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1293.2009.00814.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.7883\/yoken.JJID.2006.122","article-title":"Analysis of a long\u2010term discrepancy in drug\u2010targeted genes in plasma HIV\u20101 RNA and PBMC HIV\u20101 DNA in the same patient","volume":"59","author":"Usuku S","year":"2006","journal-title":"Jpn J Infect Dis"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq544"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e3181a5b247"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.6.2314-2321.2005"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.21.12012-12021.2004"}],"container-title":["HIV Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhiv.12170","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hiv.12170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T15:26:01Z","timestamp":1716823561000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hiv.12170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,9]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1111\/hiv.12170"],"URL":"https:\/\/doi.org\/10.1111\/hiv.12170","archive":["Portico"],"relation":{},"ISSN":["1464-2662","1468-1293"],"issn-type":[{"value":"1464-2662","type":"print"},{"value":"1468-1293","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,9]]}}}