{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:39:56Z","timestamp":1775018396896,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T00:00:00Z","timestamp":1435190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2016,1]]},"abstract":"Objectives<\/jats:title>The aim of the study was to evaluate the role of plasma K<\/jats:styled-content>aposi sarcoma herpesvirus (KSHV<\/jats:styled-content>) as a diagnostic and prognostic biomarker in people living with HIV<\/jats:styled-content> (PLWH<\/jats:styled-content>) and diagnosed with KSHV<\/jats:styled-content>\u2010associated diseases.<\/jats:p><\/jats:sec>Methods<\/jats:title>Using quantitative nested polymerase chain reaction (PCR<\/jats:styled-content>) targeting the open reading frame\u201026 gene of KSHV<\/jats:styled-content>, plasma levels of KSHV<\/jats:styled-content> were measured in consecutive PLWH<\/jats:styled-content> with KSHV<\/jats:styled-content>\u2010associated diseases or as part of the investigation of lymphadenopathy.<\/jats:p><\/jats:sec>Results<\/jats:title>Plasma KSHV<\/jats:styled-content> assays were performed on samples from 684 PLWH<\/jats:styled-content> and 20 HIV<\/jats:styled-content>\u2010seronegative people with KSHV<\/jats:styled-content>\u2010associated malignancies. In PLWH<\/jats:styled-content>, plasma KSHV<\/jats:styled-content> was detected in 39% of those with KS<\/jats:styled-content>, 99% of those with multicentric Castleman disease (MCD<\/jats:styled-content>), 9% of those with non\u2010H<\/jats:styled-content>odgkin lymphoma (NHL<\/jats:styled-content>), 2% of those with non\u2010AIDS<\/jats:styled-content>\u2010defining malignancies and 0% of those with nonmalignant lymphadenopathy. There was no significant difference in plasma KSHV<\/jats:styled-content> viral load among those with KS<\/jats:styled-content>, MCD<\/jats:styled-content> and KSHV<\/jats:styled-content>\u2010associated NHL<\/jats:styled-content>. The 5\u2010year overall survival rate from KS<\/jats:styled-content> diagnosis of 335 PLWH<\/jats:styled-content> was 95.2% (95% confidence interval 92.6\u201397.8%). Plasma KSHV<\/jats:styled-content> viraemia did not predict overall survival in those with KS<\/jats:styled-content> (P<\/jats:italic>\u2009=\u20090.73), nor when those with T0 stage KS<\/jats:styled-content> (P<\/jats:italic>\u2009=\u20090.52) or T1 stage KS<\/jats:styled-content> (P<\/jats:italic>\u2009=\u20090.62) were analysed separately.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Measuring the plasma levels of KSHV<\/jats:styled-content> as a biomarker in KSHV<\/jats:styled-content>\u2010associated disease has a very limited value in either diagnosis or prognostication. The only potential role of clinical value is the suggestion that an undetectable plasma KSHV<\/jats:styled-content> excludes a diagnosis of MCD<\/jats:styled-content> in PLWH.<\/jats:styled-content><\/jats:p><\/jats:sec>","DOI":"10.1111\/hiv.12273","type":"journal-article","created":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T10:19:27Z","timestamp":1435227567000},"page":"56-61","source":"Crossref","is-referenced-by-count":26,"title":["The clinical application of plasma K<\/scp>aposi sarcoma herpesvirus viral load as a tumour biomarker: results from 704 patients"],"prefix":"10.1111","volume":"17","author":[{"given":"I\u2010U","family":"Haq","sequence":"first","affiliation":[{"name":"Imperial College School of Medicine London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Dalla Pria","sequence":"additional","affiliation":[{"name":"Department of Oncology, National Centre for HIV Malignancies Chelsea & Westminster Hospital London 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Westminster Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Bower","sequence":"additional","affiliation":[{"name":"Imperial College School of Medicine London UK"},{"name":"Department of Oncology, National Centre for HIV Malignancies Chelsea & Westminster Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.07.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91619-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200308150-00015"},{"key":"e_1_2_6_5_1","first-page":"283","article-title":"HHV\u20108 DNA in Blood and the Development of HIV\u2010associated Kaposi's Sarcoma in the Era of HAART A Prospective Evaluation","volume":"7","author":"Lorenzen T","year":"2002","journal-title":"Eur J Med 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