{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:32:19Z","timestamp":1790789539310,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T00:00:00Z","timestamp":1633824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011318","name":"Fondation Botnar","doi-asserted-by":"publisher","award":["REG\u201019\u2010008"],"award-info":[{"award-number":["REG\u201019\u2010008"]}],"id":[{"id":"10.13039\/501100011318","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["PCEFP3_181355"],"award-info":[{"award-number":["PCEFP3_181355"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Since 2018, the World Health Organization has recommended dolutegravir (DTG)\u2010containing antiretroviral therapy (ART) for most people living with HIV. Country programmes across Africa have subsequently transitioned from other, mostly nonnucleoside reverse transcriptase inhibitor (NNRTI)\u2010based ART to DTG\u2010based ART. This study aims to assess the virological impact of programmatic transitioning to DTG\u2010based ART in Lesotho.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The prospective Dolutegravir in Real\u2010Life in Lesotho (DO\u2010REAL) cohort enrols people living with HIV initiating or transitioning to DTG\u2010based ART in Lesotho. Here, we present data from participants who transitioned from NNRTI\u2010 to DTG\u2010based ART between February and December 2020. Blood samples collected at transition and at 16\u00a0weeks\u2019 follow\u2010up (window 8\u201332\u00a0weeks) were used for viral load (VL) and resistance testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 1347 participants, follow\u2010up data was available for 1225. The majority (60%) were female, median age at transition was 47\u00a0years [interquartile range (IQR): 38\u201356], and median (IQR) time since ART initiation was 5.9\u00a0(3.5\u20139.0) years. Among those with complete VL data, the rate of viral suppression to &lt;\u00a0100\u00a0copies\/mL was 1093\/1116 (98%) before, 1073\/1116 (96%) at, and 1098\/1116 (98%) after transition. Even among those with a VL \u2265\u00a0100\u00a0copies\/mL at transition, 42\/44 (95%) achieved suppression to &lt;\u00a0100\u00a0copies\/mL at follow\u2010up. Seven participants had a VL \u2265\u00a01000\u00a0copies\/mL at follow\u2010up and did not harbour any integrase mutations associated with resistance to DTG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The high levels of viral suppression observed are encouraging regarding virological outcomes upon programmatic transitioning from NNRTI\u2010 to DTG\u2010based ART.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hiv.13189","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T01:32:39Z","timestamp":1634088759000},"page":"287-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Viral suppression after transition from nonnucleoside reverse transcriptase inhibitor\u2010 to dolutegravir\u2010based antiretroviral therapy: A prospective cohort study in Lesotho (DO\u2010REAL study)"],"prefix":"10.1111","volume":"23","author":[{"given":"Jennifer A.","family":"Brown","sequence":"first","affiliation":[{"name":"Clinical Research Unit, Department of Medicine Swiss Tropical and Public Health Institute  Basel Switzerland"},{"name":"Molecular Virology Group, Department of Biomedicine University of Basel  Basel Switzerland"},{"name":"University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bienvenu L.","family":"Nsakala","sequence":"additional","affiliation":[{"name":"SolidarMed Partnerships for Health  Maseru Lesotho"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuena","family":"Mokhele","sequence":"additional","affiliation":[{"name":"SolidarMed Partnerships for Health  Maseru Lesotho"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itumeleng","family":"Rakuoane","sequence":"additional","affiliation":[{"name":"SolidarMed Partnerships for Health  Maseru Lesotho"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Muhairwe","sequence":"additional","affiliation":[{"name":"SolidarMed Partnerships for Health  Maseru Lesotho"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Urda","sequence":"additional","affiliation":[{"name":"Molecular Virology Group, Department of Biomedicine University of Basel  Basel Switzerland"},{"name":"University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Amstutz","sequence":"additional","affiliation":[{"name":"Clinical Research Unit, Department of Medicine Swiss Tropical and Public Health Institute  Basel Switzerland"},{"name":"University of Basel  Basel Switzerland"},{"name":"Department of Infectious Diseases and Hospital Epidemiology University Hospital Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Tschumi","sequence":"additional","affiliation":[{"name":"Clinical Research Unit, Department of Medicine Swiss Tropical and Public Health Institute  Basel Switzerland"},{"name":"University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Klimkait","sequence":"additional","affiliation":[{"name":"Molecular Virology Group, Department of Biomedicine University of Basel  Basel Switzerland"},{"name":"University of Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklaus D.","family":"Labhardt","sequence":"additional","affiliation":[{"name":"Clinical Research Unit, Department of Medicine Swiss Tropical and Public Health Institute  Basel Switzerland"},{"name":"University of Basel  Basel Switzerland"},{"name":"Department of Infectious Diseases and Hospital Epidemiology University Hospital Basel  Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,10]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Interim guidelines: updated recommendations on first\u2010line and second\u2010line antiretroviral regimens and post\u2010exposure prophylaxis and recommendations on early infant diagnosis of HIV","author":"World Health Organization","year":"2018"},{"key":"e_1_2_8_3_1","volume-title":"Policy brief. Update of recommendations on first\u2010 and second\u2010line antiretroviral regimens","author":"World Health Organization","year":"2019"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2020.100573"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1215541"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0000000000000790"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1904340"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3018(20)30238-1"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1902824"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3018(20)30241-1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz256"},{"key":"e_1_2_8_12_1","volume-title":"Addendum to the national guidelines on the use of antiretroviral therapy for HIV prevention and treatment","author":"Ministry of Health, Government of Lesotho","year":"2019"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32462-0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003421"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy1131"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30710-2"},{"key":"e_1_2_8_17_1","volume-title":"28th virtual conference on retroviruses and opportunistic infections (CROI)","author":"Paton N","year":"2021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1807653"},{"key":"e_1_2_8_19_1","unstructured":"ZashR HolmesL&DisekoMet al. Update on neural tube defects with antiretroviral exposure in the Tsepamo study Botswana. 23rd International AIDS Conference virtual 6\u201310 July 2020 [Internet]. 2020. Accessed August 25 2021.https:\/\/www.natap.org\/2020\/IAC\/IAC_112.htm"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0220337"},{"key":"e_1_2_8_21_1","volume-title":"Confronting inequalities: lessons for pandemic responses from 40 years of AIDS","author":"UNAIDS","year":"2021"}],"container-title":["HIV Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hiv.13189","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/hiv.13189","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hiv.13189","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T19:35:35Z","timestamp":1692992135000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hiv.13189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,10]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1111\/hiv.13189"],"URL":"https:\/\/doi.org\/10.1111\/hiv.13189","archive":["Portico"],"relation":{},"ISSN":["1464-2662","1468-1293"],"issn-type":[{"value":"1464-2662","type":"print"},{"value":"1468-1293","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,10]]},"assertion":[{"value":"2021-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}