{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:44:12Z","timestamp":1772988252524,"version":"3.50.1"},"reference-count":67,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["HIV Medicine"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The study of bacterial diversity in human samples is crucial for developing biomarkers of health and disease. This research characterized the taxonomic and functional diversity of the anorectal bacterial microbiota in men who hae sex with men (MSM) with HIV compared to men from this group without HIV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>In July and August 2023, self\u2010collected anorectal swabs were obtained. DNA was extracted from each sample, and metagenomic sequencing was performed. With the obtained data, alpha and beta diversity, bacterial abundance, differential operational taxonomic units, and functional diversity were determined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Initially, 90 samples were collected, with 20 discarded due to having less than 200\u2009ng of DNA and 15 due to incomplete sequencing, leaving 55 samples analysed (15 HIV\u2010positive and 40 HIV\u2010negative). No significant differences were found between groups in terms of alpha diversity (Shannon index\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.45) and beta diversity (PERMANOVA\n                      <jats:italic>R<\/jats:italic>\n                      \u2009=\u2009\u22120.03).\n                      <jats:italic>Prevotella<\/jats:italic>\n                      was identified as the most abundant genus in both groups. Twelve genes were found to be more abundant in the anorectal microbiota of the HIV group, which promote bacterial growth, colonization and survival.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Alterations in the anorectal microbiota could influence the pathogenesis of HIV and its complications in this population, underscoring the need to investigate these mechanisms and explore interventions to improve health. Longitudinal studies are needed to analyse changes in the anorectal microbiota during HIV infection and its response to treatment, integrating metagenomic, clinical, and immunological data to better understand the interactions between HIV, the microbiota and host health.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hiv.70014","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T04:14:17Z","timestamp":1742271257000},"page":"785-799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Taxonomic and functional profile of the anorectal microbiota in HIV\u2010positive and HIV\u2010negative men who have sex with men, using a metagenomic approach"],"prefix":"10.1111","volume":"26","author":[{"given":"Paula Natalia","family":"Hern\u00e1ndez\u2010Trujillo","sequence":"first","affiliation":[{"name":"Faculty of Sciences Universidad Antonio Nari\u00f1o \u2013 Circunvalar  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Andrea","family":"Lopez\u2010Bar\u00f3n","sequence":"additional","affiliation":[{"name":"Faculty of Sciences Universidad Antonio Nari\u00f1o \u2013 Circunvalar  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5331-5693","authenticated-orcid":false,"given":"Gabriela","family":"Ar\u00e9valo\u2010Pinz\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Sciences, Pontificia Universidad Javeriana  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha Liliana","family":"Trujillo\u2010G\u00fciza","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Universidad Antonio Nari\u00f1o \u2013 Circunvalar  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha Isabel","family":"Gonz\u00e1lez\u2010Duque","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Universidad Antonio Nari\u00f1o \u2013 Circunvalar  Bogot\u00e1 Colombia"},{"name":"Tissue Engineering Group University of Applied Sciences and Arts Western Switzerland, Hepia, HES\u2010SO, School of Landscape, Engineering, and Architecture of Geneva  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Matilde","family":"Fl\u00f3rez","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Universidad Antonio Nari\u00f1o \u2013 Circunvalar  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego C.","family":"Franco","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Sciences, Ghent University  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Vanegas","sequence":"additional","affiliation":[{"name":"Faculty of Sciences Universidad Antonio Nari\u00f1o \u2013 Circunvalar  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/13691058.2016.1216604"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10508\u2010020\u201001856\u2010Y"},{"key":"e_1_2_10_4_1","article-title":"Discourses about condom use and non\u2010use among gay, bisexual men, and other men who have sex with men in two colombian cities","volume":"97","author":"Moncayo Quevedo JE","year":"2023","journal-title":"Rev Esp Salud 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