{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T11:41:46Z","timestamp":1776858106124,"version":"3.51.2"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,5,5]],"date-time":"2014-05-05T00:00:00Z","timestamp":1399248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Himalaya Drug Company"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Summary<\/jats:title>Objective<\/jats:title>Yashada bhasma (YB<\/jats:styled-content>) and T<\/jats:styled-content>ankana (TA<\/jats:styled-content>) are well characterized minerals used in traditional medicine for the treatment of various skin ailments. Yashada bhasma and TA<\/jats:styled-content> are a unique preparation of zinc and borax, respectively. The study was conducted to evaluate the in vitro<\/jats:italic> inhibitory effect of YB<\/jats:styled-content>, TA<\/jats:styled-content> and its combination (YBTA<\/jats:styled-content>) on P<\/jats:styled-content>ropionibacterium acne<\/jats:italic> growth and P<\/jats:styled-content>.\u00a0acne<\/jats:italic>\u2010induced inflammation.<\/jats:p><\/jats:sec>Methods<\/jats:title>The minerals were tested for anti\u2010P<\/jats:styled-content>.\u00a0acne<\/jats:italic> activity by disc diffusion and broth microdilution methods. The effect of these minerals on P<\/jats:styled-content>.\u00a0acne<\/jats:italic> induced TNF<\/jats:styled-content>\u2010\u03b1<\/jats:italic> and IL<\/jats:styled-content>\u20108 production and gene expression were studied in THP<\/jats:styled-content>\u20101 cells. In vitro<\/jats:italic> toxicity was tested on human keratinocytes (H<\/jats:styled-content>aC<\/jats:styled-content>aT<\/jats:styled-content>) and mouse embryonic fibroblasts (NIH<\/jats:styled-content>3T3) using MTT<\/jats:styled-content> assay.<\/jats:p><\/jats:sec>Results<\/jats:title>The minimum inhibitory concentrations (MIC<\/jats:styled-content> values) for YB<\/jats:styled-content>, TA<\/jats:styled-content> and YBTA<\/jats:styled-content> against P<\/jats:styled-content>.\u00a0acne<\/jats:italic> were 0.1\u00a0\u00b1\u00a00.2, 1.9\u00a0\u00b1\u00a00.5 and 0.3\u00a0\u00b1\u00a00.5\u00a0mg\u00a0mL\u22121<\/jats:sup>, respectively. YB<\/jats:styled-content>, TA<\/jats:styled-content> and YBTA<\/jats:styled-content> inhibited TNF<\/jats:styled-content>\u03b1<\/jats:italic> by 57.57%, 59.09% and 68.93% and IL<\/jats:styled-content>\u20108 production by 48.76%, 47.92% and 51.13% in P<\/jats:styled-content>.\u00a0acne\u2010<\/jats:italic>stimulated THP\u20101 cells, respectively. The CTC<\/jats:styled-content>50<\/jats:sub> values on H<\/jats:styled-content>aC<\/jats:styled-content>aT<\/jats:styled-content> and NIH3T3<\/jats:styled-content> was 17.44\u00a0\u00b1\u00a00.5 and 16.37\u00a0\u00b1\u00a00.2\u00a0\u03bcg\u00a0mL\u22121<\/jats:sup> for YB<\/jats:styled-content>, 1023.03\u00a0\u00b1\u00a04.0 and 1286.17\u00a0\u00b1\u00a04.4\u00a0\u03bcg\u00a0mL\u22121<\/jats:sup> for TA<\/jats:styled-content> and 89.12\u00a0\u00b1\u00a02.3 and 111.58\u00a0\u00b1\u00a03.5\u00a0\u03bcg\u00a0mL\u22121<\/jats:sup> for YBTA<\/jats:styled-content>, respectively.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The present study revealed the inhibitory effect of YB<\/jats:styled-content>, TA<\/jats:styled-content> and YBTA<\/jats:styled-content> on P<\/jats:styled-content>.\u00a0acne<\/jats:italic> growth and inflammation. Clinical studies have suggested the anti\u2010acne benefits of formulations containing YB<\/jats:styled-content> and TA<\/jats:styled-content>. The findings obtained from the present in vitro<\/jats:italic> studies provide evidence to support the mechanism of anti\u2010acne properties of YB<\/jats:styled-content> and TA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ics.12134","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T11:12:25Z","timestamp":1397905945000},"page":"361-368","source":"Crossref","is-referenced-by-count":17,"title":["Yashada bhasma (Z<\/scp>inc calx) and T<\/scp>ankana (B<\/scp>orax) inhibit P<\/scp>ropionibacterium acne<\/i> and suppresses acne induced inflammation in vitro<\/i>"],"prefix":"10.1111","volume":"36","author":[{"given":"R.","family":"Sandeep Varma","sequence":"first","affiliation":[{"name":"Department of Cell Biology Research and Development The Himalaya Drug Company Bangalore 562 162 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Shamsia","sequence":"additional","affiliation":[{"name":"Department of Microbiology Research and Development The Himalaya Drug Company Bangalore 562 162 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. S.","family":"Thiyagarajan","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Research and Development The Himalaya Drug Company Bangalore 562 162 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Vidyashankar","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Research and Development The Himalaya Drug Company Bangalore 562 162 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. S.","family":"Patki","sequence":"additional","affiliation":[{"name":"Medical Services and Clinical Trials Research and Development The Himalaya Drug Company Bangalore 562 162 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000087011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"3158","DOI":"10.1128\/iai.63.8.3158-3165.1995","article-title":"Induction of pro\u2010inflammatory cytokines by a soluble factor of Propionibacterium acnes. Implication for chronic inflammatory acne","volume":"63","author":"Vowels B.R.","year":"1995","journal-title":"Infect. 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