{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:19:16Z","timestamp":1791058756853,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T00:00:00Z","timestamp":1406246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Natura Brasil"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Synopsis<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Organotypic skin models are powerful tools for research in development, ageing and diseases. They have become more and more complex with the use of multiple cell types. This requires a culture medium adapted to optimize the development of such <jats:italic>in vitro<\/jats:italic> skin. Foetal bovine serum (<jats:styled-content style=\"fixed-case\">FBS<\/jats:styled-content>) is the most complete supplement in existence at the moment, providing at once growth factors, vitamins, hormones and other circulating compounds. However, this cocktail suffers from batch variability and its animal origin is ethically questionable. More importantly, its biological activities may interfere with the study of certain signalling pathways. Here, we present a strategy for constructing an epidermal equivalent using a defined culture medium without serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An epidermal equivalent was constructed with primary human keratinocytes cultured using an insulin\u2013transferrin\u2013selenium (<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>) medium. Determination of steady\u2010state gene expression levels and the immunohistological characterization of keratinocyte markers were performed to compare the <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> medium condition with a reference model, where keratinocytes were co\u2010cultured with fibroblasts in the presence of <jats:styled-content style=\"fixed-case\">FBS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The data show that the <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> medium promoted the expression of keratinocyte proliferation and differentiation markers at the protein and transcript levels in a similar way to that of the reference model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We show that culture using the <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content> medium appears as a viable replacement for <jats:styled-content style=\"fixed-case\">FBS<\/jats:styled-content> in the construction of epidermal equivalents, opening the way to signal transduction studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ics.12141","type":"journal-article","created":{"date-parts":[[2014,5,21]],"date-time":"2014-05-21T16:59:14Z","timestamp":1400691554000},"page":"427-435","source":"Crossref","is-referenced-by-count":23,"title":["Insulin\u2013transferrin\u2013selenium as an alternative to foetal serum for epidermal equivalents"],"prefix":"10.1111","volume":"36","author":[{"given":"C.","family":"Mainzer","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie Tissulaire et Ing\u00e9nierie Th\u00e9rapeutique CNRS UMR5305 Universit\u00e9 Lyon 1 7 passage du Vercors 69367 Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Barrichello","sequence":"additional","affiliation":[{"name":"Natura Innovation et Technologie de Produits SAS 10 rue Chevreul 92150 Suresnes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Debret","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Tissulaire et Ing\u00e9nierie Th\u00e9rapeutique CNRS UMR5305 Universit\u00e9 Lyon 1 7 passage du Vercors 69367 Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Remou\u00e9","sequence":"additional","affiliation":[{"name":"Natura Innovation et Technologie de Produits SAS 10 rue Chevreul 92150 Suresnes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Sigaudo\u2010Roussel","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Tissulaire et Ing\u00e9nierie Th\u00e9rapeutique CNRS UMR5305 Universit\u00e9 Lyon 1 7 passage du Vercors 69367 Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sommer","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Tissulaire et Ing\u00e9nierie Th\u00e9rapeutique CNRS UMR5305 Universit\u00e9 Lyon 1 7 passage du Vercors 69367 Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-006-0709-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2008.03.012"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsymp.5650015"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00545.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2010.00786.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2006.12.005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(92)90056-6"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90540-1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.14573\/altex.2010.1.53"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2005.09.006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1092"},{"key":"e_1_2_8_13_1","first-page":"332","article-title":"Effect of supplementation of dermal fibroblasts conditioned medium on expansion of keratinocytes through enhancing attachment","volume":"50","author":"Chowdhury S.R.","year":"2012","journal-title":"Indian J. 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