{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T09:23:20Z","timestamp":1696411400953},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,10,3]],"date-time":"2016-10-03T00:00:00Z","timestamp":1475452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007608","name":"AgResearch","doi-asserted-by":"publisher","award":["A14811"],"award-info":[{"award-number":["A14811"]}],"id":[{"id":"10.13039\/100007608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Exposure to <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> in humans resulting in sunburn triggers a complex series of events that are a mix of immediate and delayed damage mediation and healing. While studies on the effects of <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> exposure on <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage and repair have been reported, changes in the oxidative modification of skin proteins are poorly understood at the molecular level, despite the important role played by structural proteins in skin tissue, and the effect of the integrity of these proteins on skin appearance and health. Proteomic molecular mapping of oxidation was here applied to try to enhance understanding of skin damage and recovery from oxidative damage and <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A redox proteomic\u2010based approach was applied to evaluating skin protein modification when exposed to varying doses of <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> after initial oxidative stress, via tracking changes in protein oxidation during the healing process <jats:italic>in vitro<\/jats:italic> using a full\u2010thickness reconstituted human skin tissue model. Bioassays and structural evaluation confirmed that our cultured skin tissues underwent a normal physiological response to <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A set of potential skin marker peptides was generated, for use in tracking skin protein oxidative modification. Exposure to <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> after thermal oxidative stress was found to result in higher levels of skin protein oxidation than a non\u2010irradiated control for up to seven days after exposure. Recovery of the skin proteins from oxidative stress, as assessed by the overall protein oxidation levels, was found to be impaired by <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> exposure. Oxidative modification was largely observed in skin structural proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Exposure of skin proteins to <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> exacerbates oxidative damage to structural skin proteins, with higher exposure levels leading to increasingly impaired recovery from this damage. This has potential implications for the functional performance of the proteins and inter\u2010related skin health and cosmetic appearance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ics.12365","type":"journal-article","created":{"date-parts":[[2016,9,10]],"date-time":"2016-09-10T14:17:18Z","timestamp":1473517038000},"page":"197-205","source":"Crossref","is-referenced-by-count":2,"title":["Redox proteomic evaluation of oxidative modification and recovery in a 3D reconstituted human skin tissue model exposed to <scp>UVB<\/scp>"],"prefix":"10.1111","volume":"39","author":[{"given":"J. M.","family":"Dyer","sequence":"first","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"},{"name":"Riddet Institute, Massey University Private Bag 11222 Palmerston North 4442 New Zealand"},{"name":"Biomolecular Interaction Centre University of Canterbury Private Bag 4800 Christchurch 8140 New Zealand"},{"name":"Wine, Food &amp; Molecular Biosciences PO Box 85084, Lincoln University, Lincoln 7647 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Haines","sequence":"additional","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Wang","sequence":"additional","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Walls","sequence":"additional","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Clerens","sequence":"additional","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"},{"name":"Biomolecular Interaction Centre University of Canterbury Private Bag 4800 Christchurch 8140 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Harland","sequence":"additional","affiliation":[{"name":"Food &amp; Bio\u2010Based Products AgResearch Ltd Private Bag 4749, Christchurch 8140 New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/B504970A"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2494.2008.00454.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1993.tb04981.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0030-6665(20)30862-8","article-title":"Effects of solar radiation on the skin","volume":"26","author":"Buzzell R.A.","year":"1993","journal-title":"Otolaryngol. Clin. North Am."},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(81)70103-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-007-0804-3"},{"key":"e_1_2_6_8_1","first-page":"127","article-title":"The sunburn cell","volume":"4","author":"Young A.R.","year":"1987","journal-title":"Photodermatology"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09449.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(03)00150-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0047-6374(01)00225-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01708.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1996.d01-1088.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(00)00098-X"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-5896-4_8"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12327.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23597.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12076"},{"key":"e_1_2_6_19_1","first-page":"246","article-title":"EpiDerm\u2010FT: a full\u2010thickness in vitro human skin equivalent for wound healing and toxicology applications","volume":"40","author":"Hayden P.J.","year":"2004","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/aivt.2015.0010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2013.0293"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2009.11.008"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2009.00614.x"}],"container-title":["International Journal of Cosmetic Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fics.12365","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fics.12365","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ics.12365","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:59:58Z","timestamp":1696319998000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ics.12365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,3]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1111\/ics.12365"],"URL":"https:\/\/doi.org\/10.1111\/ics.12365","archive":["Portico"],"relation":{},"ISSN":["0142-5463","1468-2494"],"issn-type":[{"value":"0142-5463","type":"print"},{"value":"1468-2494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,3]]}}}