{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T21:32:47Z","timestamp":1768339967100,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T00:00:00Z","timestamp":1561334400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In order to comply with the European legislation concerning the risk assessment of skin sensitizers, considerable progress has been made in developing alternative methods, such as human cell line activation test (h\u2010<jats:styled-content style=\"fixed-case\">CLAT<\/jats:styled-content>). H\u2010<jats:styled-content style=\"fixed-case\">CLAT<\/jats:styled-content>is based on cytometric measurement of fluorescence emitted by anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>54 and anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>86 antibodies in<jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101 cells. Following this method, a range of substances have been analyzed; the emitted fluorescence, generally at low intensity, has caused problems concerning the interpretation of results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>Find an alternative parameter to h\u2010<jats:styled-content style=\"fixed-case\">CLAT<\/jats:styled-content>for evaluating the sensitizing potential of chemicals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Cells have been analyzed with flow cytometry after treatment with sensitizing compounds administered at non\u2010cytotoxic concentrations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sensitizers were able to inducealterations in cell morphology to a more \u2018condensed\u2019 one allowing the identification of cells under microscope as a \u2018sensitized\u2019 subpopulation. These variations cause similar modifications in \u2018scattering\u2019 parameters, making cells easily monitorable by flow cytometry. No changes have been observed in cells treated with non\u2010sensitizers or in untreated cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This method based on the analysis of forward scatter and side scatter parameters, can be used as an alternative method for identifying sensitization potential of chemical compounds.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ics.12530","type":"journal-article","created":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T00:17:16Z","timestamp":1555460236000},"page":"257-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A preliminary study of an alternative method for evaluating skin sensitizing potential of chemicals"],"prefix":"10.1111","volume":"41","author":[{"given":"A.","family":"Buzzella","sequence":"first","affiliation":[{"name":"Department of Biology and Biotechnology \u201cLazzaro Spallanzani\u201d University of Pavia Pavia Italy"},{"name":"BioBasic Europe s.r.l. Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Mazzini","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology \u201cLazzaro Spallanzani\u201d University of Pavia Pavia Italy"},{"name":"Institute of Molecular Genetics CNR Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Vicini","sequence":"additional","affiliation":[{"name":"BioBasic Europe s.r.l. Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Angelinetta","sequence":"additional","affiliation":[{"name":"BioBasic Europe s.r.l. Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Pastoris","sequence":"additional","affiliation":[{"name":"Department of Biology and Biotechnology \u201cLazzaro Spallanzani\u201d University of Pavia Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,24]]},"reference":[{"issue":"9","key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1111\/ddg.13128","article-title":"New developments in occupational dermatology","volume":"14","author":"Diepgen T.L.","year":"2016","journal-title":"J. Dtsch Dermatol. Ges."},{"issue":"5","key":"e_1_2_6_3_1","first-page":"284","article-title":"Allergic contact dermatitis","volume":"14","author":"Saint\u2010Mezard P.","year":"2004","journal-title":"Eur. J. Dermatol."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00173-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02082.x"},{"key":"e_1_2_6_6_1","unstructured":"OECD Series on Testing and Assessment No. 168. The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins. Part 1: Scientific Evidence. OECD 2012. Available at:http:\/\/www.oecd.org\/officialdocuments\/publicdisplaydocumentpdf\/?cote=ENV\/JM\/MONO(2012)10\/PART1&docLanguage=En."},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx7002239"},{"key":"e_1_2_6_8_1","unstructured":"EU Cosmetic Regulations \u2010 76\/768\/EEC the new EU cosmetic products regulation (EC) No 1223\/2009."},{"key":"e_1_2_6_9_1","unstructured":"OECD.OECD 442E. Key event\u2010based test guideline. 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