{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:26:36Z","timestamp":1783117596596,"version":"3.54.6"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T00:00:00Z","timestamp":1648339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Hair loss and greying affect men and women of all ages, often causing psychosocial difficulties. Dickkopf\u20101 (DKK1), a major hair loss factor secreted from dermal papilla (DP) cells in response to the secretion of dihydrotestosterone (DHT), has been reported to induce and accelerate androgenetic alopecia (AGA). In addition, DKK1 acts as a potent suppressor of melanogenesis and is closely related to hair colour. R\u2010spondin 1 (RSPO1) is a secretory agonist of Wnt signalling known to antagonize the effects of DKK1, including DKK1\u2010mediated hair follicle suppression. In this study, we investigated the effect of watercress extract (WCE) on the secretion of RSPO1 and DKK1 from DP cells as well as its anti\u2010hair loss effect in human hair follicles and patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:italic>in vitro<\/jats:italic> secretion of RSPO1 and DKK1 was measured by ELISA. Human hair follicles were collected from the scalp of a female donor and used for <jats:italic>ex vivo<\/jats:italic> organ culture to investigate the effects of WCE on human hair loss. Finally, a 6\u2010month human clinical trial was conducted to examine the effect of WCE\u2010containing lotion on hair growth in a male panel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>WCE significantly upregulated RSPO1\u00a0secretion and suppressed DKK1\u00a0secretion in a dose\u2010dependent manner, even in the presence of DHT. WCE\u2010treated hair follicles elongated 1.6\u2010fold compared with the control, and the level of RSPO1 production in DP as well as RSPO1 bound to the outer root sheath (ORS) increased. In the clinical trial, the hair lotion containing 2% WCE increased hair thickness and density to improve against hair loss symptoms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>WCE exhibited a strong anti\u2010androgenic effect through its ability to suppress DKK1\u00a0secretion and antagonize DKK1 via RSPO1. These findings highlighted the potential use of WCE for the treatment of hair loss.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ics.12764","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T16:56:26Z","timestamp":1644339386000},"page":"154-165","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of watercress extract fraction on R\u2010spondin 1\u2010mediated growth of human hair"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3184-9093","authenticated-orcid":false,"given":"Masakazu","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Research and Development Department Ichimaru Pharcos Co., Ltd  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Kawai","sequence":"additional","affiliation":[{"name":"Research and Development Department Ichimaru Pharcos Co., Ltd  Gifu 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