{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T19:59:54Z","timestamp":1776110394926,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T00:00:00Z","timestamp":1691971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Intensive studies have revealed that pleiotropic melanocytic factors are associated with age\u2010spot formation. Dysfunctional keratinocyte differentiation is thought to be an upstream cause of age\u2010spot formation. Although it has been shown that keratinocyte differentiation is mediated by the cell\u2013cell contact factor E\u2010cadherin, its involvement in age\u2010spot formation remains unknown. Thus, to determine the origin of age\u2010spots and an integrated solution for the same, we focused on E\u2010cadherin expression in the present study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>First, we assessed the solar lentigines in cutaneous and cultured cells by means of immunofluorescence staining. Following that, keratinocytes treated with siRNAs against E\u2010cadherin were co\u2010cultured with melanocytes, and the secreted factors were identified by means of proteomic analysis of the culture supernatants. We also performed quantitative PCR to assess melanogenesis activity and screen ingredients. For behavioural analysis of melanocytes, we performed time\u2010lapse imaging using confocal laser scanning microscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>E\u2010cadherin expression was downregulated in the epidermis of the solar lentigines, suggesting its involvement in age\u2010spot formation. E\u2010cadherin knocked down keratinocytes not only promoted the secretion of melanocytic\/inflammatory factors but also increased melanogenesis by upregulating the expression of melanogenesis factors. Furthermore, live\u2010imaging showed that the downregulation of E\u2010cadherin inhibited melanocyte dynamics and accelerated melanin uptake. Finally, we identified Rosa multiflora fruit extract as a solution that can upregulate E\u2010cadherin expression in keratinocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings showed that E\u2010cadherin downregulation triggers various downstream melanocytic processes, such as the secretion of melanocytic factors and melanogenesis. Additionally, we showed that the Rosa multiflora fruit extract upregulated E\u2010cadherin expression in keratinocytes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ics.12886","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T07:09:01Z","timestamp":1690787341000},"page":"775-790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A mechanism of melanogenesis mediated by E\u2010cadherin downregulation and its involvement in solar lentigines"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0714-861X","authenticated-orcid":false,"given":"Daigo","family":"Inoue","sequence":"first","affiliation":[{"name":"Shiseido Co. Ltd. MIRAI Technology Institute  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Narita","sequence":"additional","affiliation":[{"name":"Shiseido Co. Ltd. MIRAI Technology Institute  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Ono","sequence":"additional","affiliation":[{"name":"Shiseido Co. Ltd. MIRAI Technology Institute  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Shiseido Co. Ltd. MIRAI Technology Institute  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki","family":"Maeno","sequence":"additional","affiliation":[{"name":"Shiseido Co. Ltd. MIRAI Technology Institute  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirofumi","family":"Aoki","sequence":"additional","affiliation":[{"name":"Shiseido Co. Ltd. MIRAI Technology Institute  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Motoyama","sequence":"additional","affiliation":[{"name":"Shiseido Co. 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