{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T14:17:06Z","timestamp":1788617826264,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intern J of Cosmetic Sci"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the role of glycerol and urea in water\u2010holding mechanisms in the stratum corneum (SC) using synchrotron X\u2010ray diffraction and to clarify differences in activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Structural changes in human SC samples treated with water, glycerol, and urea aqueous solutions were measured during drying, focusing on the short lamellar, orthorhombic hydrocarbon\u2010chain packing, and coiled\u2010coil \u03b1\u2010helix structure of the soft keratin, which are affected by water.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>During drying of the treated SC, the coiled\u2010coil \u03b1\u2010helix chain distance decreased due to the removal of bound water. The reduction rate was suppressed in the SC treated with glycerol, followed by those treated with urea. This result suggests that water binding via glycerol is strongest to soft keratin, followed by urea. Furthermore, for the short lamellar structure, we calculated the volume associated with the hydrocarbon chain, its head group, and water in the head group, from which we estimated the quantity of water removal. Initially, the volume was unchanged because no water was removed. Once the SC was treated with glycerol and urea, the volume\u2010unchanged interval was longer than that in the SC treated with water alone. Therefore, glycerol and urea strengthened water retention within the water layer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken together with the results obtained from the solid\u2010state NMR analysis of the soft keratin, the present results indicate that glycerol enhances the retention of the maximum bound water of 25\u2009wt% in the SC, whereas urea retains it somewhat weakly. Therefore, from the results of in\u00a0vivo confocal Raman microscopy, which indicated that the water content near the surface of the normal skin is approximately 25\u2009wt%, it is deduced that the maximum bound water content is strongly held at the skin surface with the aid of glycerol or urea. On the other hand, it was found that the lipid lamellar structure serves to regulate the water content to maintain it at 25\u2009wt% and glycerol and urea work to enhance this regulation mechanism. Owing to the above two mechanisms, the water content at the skin surface is maintained at 25\u2009wt% homeostatically, and the homeostatic state is strengthened by glycerol and urea.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ics.13069","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:33:42Z","timestamp":1747802022000},"page":"864-876","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enhancement of water homeostasis by glycerol and urea in stratum corneum and the difference in the activity between them"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7070-7849","authenticated-orcid":false,"given":"Ryosuke","family":"Toyoshima","sequence":"first","affiliation":[{"name":"Sakamoto Yakuhin Kogyo Co., Ltd.  Izumi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Murashima","sequence":"additional","affiliation":[{"name":"Sakamoto Yakuhin Kogyo Co., Ltd.  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