{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:01:29Z","timestamp":1785283289890,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Intern J of Cosmetic Sci"],"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n To evaluate the clinical efficacy of a piroctone olamine\u2013containing shampoo in individuals with oily dandruff and to characterize associated temporal changes in bacterial and fungal scalp microbiota.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n An open\u2010label, single\u2010arm, longitudinal study was conducted in 41 volunteers with oily dandruff over 28\u2009days. Participants applied the test shampoo three times weekly. Clinical assessments (erythema, pruritus, desquamation index and sebum levels) were performed at baseline, Day 14, and Day 28. Scalp microbiome samples were collected by standardized swabbing and analyzed using full\u2010length 16S rRNA gene and ITS amplicon sequencing. Microbial community dynamics were evaluated using compositional data analysis, including centred log\u2010ratio transformation, robust Aitchison distance, compositional tensor factorization and linear mixed\u2010effects modelling.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Significant clinical improvement was observed by Day 28, with marked reductions in erythema and pruritus. Sebum levels increased during the study period (\n p<\/jats:italic>\n \u2009<\u20090.01), indicating that symptom improvement occurred independently of reduced sebum production. Bacterial community analysis revealed progressive restructuring of the scalp microbiome, becoming most evident by Day 28. This shift was characterized by a descriptive increase in the relative abundance of\n Cutibacterium acnes<\/jats:italic>\n together with a numerical decline in\n Staphylococcus capitis<\/jats:italic>\n , resulting in an increase in the\n C. acnes\/Staphylococcus<\/jats:italic>\n ratio from 2.83 to 4.51. Longitudinal compositional analyses further identified several bacterial genera associated with the temporal shift, including\n Streptococcus<\/jats:italic>\n ,\n Flavobacterium<\/jats:italic>\n and\n Sphingomonas<\/jats:italic>\n . The fungal community responded earlier to treatment, showing directional reduction of\n Malassezia<\/jats:italic>\n \u2010associated dominance together with enrichment of several non\u2010\n Malassezia<\/jats:italic>\n genera. Fungal richness increased significantly by Day 14 and remained elevated at Day 28, while beta\u2010diversity analyses confirmed significant temporal restructuring of both bacterial and fungal communities.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Use of a piroctone olamine shampoo was associated with significant clinical improvement and measurable shifts in both bacterial and fungal scalp microbiota. These findings suggest that dandruff improvement may coincide with ecological restructuring of the scalp microbiome rather than broad microbial suppression.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ics.70128","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T23:53:56Z","timestamp":1785282836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical improvement and scalp microbiome restructuring following a 28\u2010day piroctone olamine shampoo intervention"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1532-9397","authenticated-orcid":false,"given":"Spyridon","family":"Markos","sequence":"first","affiliation":[{"name":"QACS Ltd., Quality Assurance & Control Systems Metamorfosi Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Praefke","sequence":"additional","affiliation":[{"name":"Sebapharma GmbH & Co. KG Boppard Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiannis","family":"Kapetanstatakis","sequence":"additional","affiliation":[{"name":"QACS Ltd., Quality Assurance & Control Systems Metamorfosi Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michail","family":"Tsanakas","sequence":"additional","affiliation":[{"name":"Consultant Dermatologist Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelos","family":"Beletsiotis","sequence":"additional","affiliation":[{"name":"QACS Ltd., Quality Assurance & Control Systems Metamorfosi Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"issue":"2","key":"e_1_2_9_2_1","article-title":"Seborrheic dermatitis and dandruff: a comprehensive review","volume":"3","author":"Borda LJ","year":"2015","journal-title":"J Clin Investig 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