{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:05:40Z","timestamp":1789445140388,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dental Hygiene"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to investigate the clinical and biochemical effects of repeated injectable platelet\u2010rich fibrin (i\u2010PRF) application into periodontal pockets following scaling and root planing (SRP) in patients with periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Twenty patients with Stage III Grade B periodontitis were enrolled in this randomized, controlled, split\u2010mouth study. i\u2010PRF was applied into all periodontal pockets at the test site after SRP and repeated after 2\u2009weeks, whereas the control site received only sterile saline irrigation. Clinical periodontal parameters were recorded at baseline and at 3\u2009months. Gingival crevicular fluid (GCF) samples were collected at baseline, on Day 7 following each i\u2010PRF application, and at 3\u2009months. Levels of Platelet Factor\u20104 (PF4), Platelet\u2010Derived Growth Factor\u2010BB (PDGF\u2010BB), Transforming Growth Factor\u2010\u03b21 (TGF\u2010\u03b21), and Myeloperoxidase (MPO) were analysed using the enzyme\u2010linked immunosorbent assay (ELISA) method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In periodontal pockets with an initial depth of \u2265\u20094\u2009mm, no statistically significant differences were observed between the groups in probing depth (PD) or bleeding on probing (BOP) at 3\u2009months (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). However, clinical attachment loss (CAL), Plaque Index (PI), and Gingival Index (GI) values were significantly lower in the test sites than the control sites at 3\u2009months (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05, and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05, respectively). TGF\u2010\u03b21 total amounts were higher in the test sites at Week 2 and at Month 3 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). MPO total amounts were higher in the test sites at Week 4 compared to control sites (\n                      <jats:italic>p\u2009&lt;<\/jats:italic>\n                      \u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Adjunctive use of i\u2010PRF with SRP may enhance tissue healing and improve periodontal clinical parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      NCT07029035, 19 June 2025 (retrospectively registered)\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/idh.70162","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:50:56Z","timestamp":1789444256000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical and Biochemical Effects of Repeated Injectable Platelet\u2010Rich Fibrin Application in Periodontitis Treatment: A Randomized Controlled Clinical Trial"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6804-3877","authenticated-orcid":false,"given":"Berrak","family":"Ulu Emir","sequence":"first","affiliation":[{"name":"Department of Periodontology, Gulhane Faculty of Dentistry University of Health Sciences  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5633-9359","authenticated-orcid":false,"given":"Erkan","family":"\u00d6zcan","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Gulhane Faculty of Dentistry University of Health Sciences  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1046-8208","authenticated-orcid":false,"given":"Emre","family":"Avc\u0131","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Gulhane Faculty of Pharmacy University of Health Sciences  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"Newman and Carranza's Clinical Periodontology","author":"Preshaw P. 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