{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:18:21Z","timestamp":1785759501937,"version":"3.56.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the expression of leptin in healthy and inflamed human dental pulp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Twenty\u2010one pulp samples were obtained from freshly caries\u2010 and restoration\u2010free extracted human third molars. In seven\u2010third molars (inflamed pulp group), inflammation was induced prior to extraction. Pulp samples were processed, and leptin expression was determined by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010PCR) and the amount of leptin by immunoblot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All healthy and inflamed dental pulp samples expressed leptin. Western blot analysis revealed the presence of a protein with an apparent molecular weight of ~16 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a in human dental pulp, which corresponds to the estimated molecular weight of leptin. The expression of leptin <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> in dental pulp was confirmed by <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010PCR analysis, and the size of the amplified fragments (296 bp for leptin and 194\u00a0bp for cyclophilin) was confirmed by agarose gel electrophoresis. The expression of leptin in the inflamed pulp group was significantly greater (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) than in healthy teeth. The relative amount of leptin in inflamed pulps was almost twice than in healthy pulps.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For the first time, the presence of leptin in human dental pulp tissues has been demonstrated. The upregulation of leptin expression in inflamed pulp samples suggests that leptin can play a role in pulpal inflammatory and immune responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12009","type":"journal-article","created":{"date-parts":[[2012,9,13]],"date-time":"2012-09-13T09:45:08Z","timestamp":1347529508000},"page":"442-448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Leptin expression in healthy and inflamed human dental pulp"],"prefix":"10.1111","volume":"46","author":[{"given":"J.","family":"Mart\u00edn\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Department of Stomatology (Endodontics section) School of Dentistry, University of Sevilla, Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"S\u00e1nchez\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena Hospital, University of Sevilla, Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"P\u00e9rez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena Hospital, University of Sevilla, Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Carmona\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena Hospital, University of Sevilla, Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"S\u00e1nchez\u2010Margalet","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena Hospital, University of Sevilla, Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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