{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:20:47Z","timestamp":1772209247381,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,1,21]],"date-time":"2013-01-21T00:00:00Z","timestamp":1358726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate whether the apoptotic cascade is activated through the extrinsic pathway in epithelial lining and connective tissue of radicular cysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Fifteen radicular cysts were fixed in formalin, embedded in paraffin wax and processed for immunohistochemistry to evaluate the expression of polyclonal antibodies against Tumour necrosis factor\u2013related apoptosis\u2010inducing ligand (<jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>5 and caspase\u20103. Immunocomplexes were treated with the secondary antibodies and finally detected using the avidin\u2013biotin\u2013peroxidase complex. Immunoreactivity was visualized by development with 3,3\u2032\u2010diaminobenzidine. Data were analysed using the Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2010test; <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05 was considered significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The three antibodies were detected in connective tissue fibroblasts of all radicular cysts; <jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>5 immunoexpression was significantly greater (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) compared with that of caspase\u20103. The three antibodies were also expressed in almost all epithelial layers and in endothelial cells of newly formed vessels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The involvement of apoptosis in the pathogenesis of radicular cysts, demonstrated by the immunoexpression patterns of <jats:styled-content style=\"fixed-case\">TRAIL<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> DR<\/jats:styled-content>5 and caspase\u20103 in lining epithelium and connective tissue, may explain their bland clinical aggressiveness and slow, benign evolution.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12040","type":"journal-article","created":{"date-parts":[[2012,11,21]],"date-time":"2012-11-21T06:57:00Z","timestamp":1353481020000},"page":"642-648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Possible role of apoptosis in the pathogenesis and clinical evolution of radicular cyst: an immunohistochemical study"],"prefix":"10.1111","volume":"46","author":[{"given":"C.","family":"Loreto","sequence":"first","affiliation":[{"name":"Department of Bio\u2010Medical Sciences Section of Anatomy University of Catania Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Galanti","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Catania Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Leonardi","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Catania Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Musumeci","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010Medical Sciences Section of Anatomy University of Catania Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Pannone","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences Institute of Pathology and Cytopathology University of Foggia Foggia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Palazzo","sequence":"additional","affiliation":[{"name":"Department of Orthodontics University of Catania Catania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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