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Dycal was used as a positive control for the cell viability assay. Teflon rings were cocultured with <jats:styled-content style=\"fixed-case\">hTGSC<\/jats:styled-content>s, followed by the induction of odontogenic differentiation. The odontogenic differentiation of <jats:styled-content style=\"fixed-case\">hTGSC<\/jats:styled-content>s and biomineralization ability of the materials were evaluated by analysing the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression levels of dentine sialophosphoprotein (DSPP) and collagen type 1A (COL1A) by real\u2010time polymerase chain reaction expression analysis, measurement of alkaline phosphatase (ALP) activity and visualization of calcium deposits by von Kossa staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MTA and <jats:styled-content style=\"fixed-case\">iR<\/jats:styled-content>oot SP exhibited no cytotoxicity, but Dycal caused cytotoxicity (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) of almost all of the cells after 7\u00a0days. MTA significantly stimulated (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) the odontogenic differentiation of <jats:styled-content style=\"fixed-case\">hTGSC<\/jats:styled-content>s compared with <jats:styled-content style=\"fixed-case\">iR<\/jats:styled-content>oot SP. MTA and <jats:styled-content style=\"fixed-case\">iR<\/jats:styled-content>oot SP increased (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of COL1A and DSPP <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> compared with noninduced <jats:styled-content style=\"fixed-case\">hTGSC<\/jats:styled-content>s, which served as a negative control (NC). <jats:styled-content style=\"fixed-case\">iR<\/jats:styled-content>oot SP, however, significantly decreased (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) COL1A and DSPP <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression levels compared with the PC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MTA and <jats:styled-content style=\"fixed-case\">iR<\/jats:styled-content>oot SP induced <jats:styled-content style=\"fixed-case\">hTGSC<\/jats:styled-content> differentiation into odontoblast\u2010like cells, but MTA might provide more inductive potential and hard tissue deposition compared with <jats:styled-content style=\"fixed-case\">iR<\/jats:styled-content>oot SP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12115","type":"journal-article","created":{"date-parts":[[2013,4,19]],"date-time":"2013-04-19T10:56:43Z","timestamp":1366369003000},"page":"1173-1182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["<i>In vitro<\/i> comparison of induction capacity and biomineralization ability of mineral trioxide aggregate and a bioceramic root canal sealer"],"prefix":"10.1111","volume":"46","author":[{"given":"E. 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