{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T15:05:04Z","timestamp":1696345504564},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T00:00:00Z","timestamp":1395273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the kinetics of <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 and p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9) expression in experimentally induced rat periapical lesions and to explore their possible functions in the pathogenesis of periapical lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Periapical lesions were established in Wistar rats by occlusal pulp exposure in mandibular first molar teeth. The animals were killed on days 0, 7, 14, 21 and 28. Micro\u2010computed tomographic, histological and enzyme histochemical analyses were performed to detect the progression of periapical lesions. Immunohistochemistry, double\u2010dye immunofluorescence and Western blot were performed to determine the expression of <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 and p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9) in periapical tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>From day 0 to day 28, the lesion volume and area gradually expanded, and the <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2\u2010positive cells gradually ascended. A few p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9)\u2010positive cells and osteoclasts appeared on day 7 and then climaxed on day 14. The numbers then simultaneously decreased from day 21 to day 28. Western blot analysis revealed that p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9) and <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 proteins were expressed at all time\u2010points. The positive cells and protein expression ratio of p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9) against <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 increased from day 0 to day 14 and then decreased from day 14 to day 28. Finally, double\u2010dye immunofluorescence assay revealed that p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9)\u2010positive and <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content>\u2010positive cells were co\u2010localized around periapical lesions on days 14 and 28.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 and p\u2010<jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3\u03b2 (Ser 9) can be observed and may be involved in alveolar bone resorption and inflammatory response in periapical lesions, as well as associated with periapical lesion pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12258","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T23:17:17Z","timestamp":1391555837000},"page":"1107-1116","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Kinetics of glycogen synthase kinase (<scp>GSK<\/scp>)3\u03b2 and phosphorylated <scp>GSK<\/scp>3\u03b2 (Ser 9) expression in experimentally induced periapical lesions"],"prefix":"10.1111","volume":"47","author":[{"given":"Z.","family":"Sun","sequence":"first","affiliation":[{"name":"School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry and Endodontics School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry and Endodontics School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2012.00180"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.08.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2002.00467.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790010401"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2009.09.007"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1415"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.03.005"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00384"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000178350.21839.44"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2008.02319.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.256768"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2003.12.004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-006-9128-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(96)80266-4"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2006.11.036"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2003.00627.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1221"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302x.2000.150301.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101254"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(98)00068-4"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130401500604"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/327417"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2008.00085.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680051601"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(94)90044-2"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090040701"},{"issue":"1","key":"e_1_2_7_28_1","first-page":"S137","article-title":"Glycogen synthase kinase 3 (GSK3) in the heart: a point of integration in hypertrophic signalling and a therapeutic target? 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