{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T05:44:45Z","timestamp":1768715085804,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,4,4]],"date-time":"2014-04-04T00:00:00Z","timestamp":1396569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FAPESP - Research assistance","award":["2011\/14385-6"],"award-info":[{"award-number":["2011\/14385-6"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the absence of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 on the progression of periapical lesions in wild\u2010type (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 knockout (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content>) mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The evaluation of the oral microbial profile of mice was performed by Checkerboard <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> hybridization from saliva samples. Periapical lesions were induced in manbibular first molars by pulpal exposure and evaluated after 7, 21 and 42\u00a0days (<jats:italic>n<\/jats:italic>\u00a0=\u00a015). Haematoxylin\u2010eosin\u2010stained sections were analysed under conventional and fluorescence microscopy to evaluate the tissue features and size of periapical lesions and tartrate\u2010resistant acid phosphatase histoenzymology (<jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content>), Brown &amp; Brenn staining and immunohistochemistry. The scores of the number of bacterial cells present in the oral cavity were analysed by the Mann\u2013Whitney test, and the results and comparisons for periapical lesion size and number of osteoclasts were subjected to one\u2010way <jats:sc>anova<\/jats:sc> and Bonferroni's post\u2010test (\u03b1\u00a0=\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant differences were observed for bacterial load between the groups of animals for 6 bacterial species (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), with five species found in higher levels in the <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> group, and one in the <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> group. <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> mice had significantly larger periapical lesions (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) between 7 and 42\u00a0days and between 21 and 42\u00a0days, with an increase in the mean size and number of osteoclasts. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice had an increase in periapical lesion size and number of osteoclasts between 7 and 21\u00a0days (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). No differences were found between bacteria localization in the root canal system between the experimental groups. Small variations related to the location of immunostaining were found between the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study revealed differences in the composition of oral microbiota between mice that may be taken into account in the susceptibility to infections and development of periapical lesions. The absence of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 in mice resulted in smaller periapical lesions with fewer osteoclasts at the final experimental period, suggesting the participation of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 in the host immune and inflammatory response to a periradicular infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12274","type":"journal-article","created":{"date-parts":[[2014,2,26]],"date-time":"2014-02-26T06:01:30Z","timestamp":1393394490000},"page":"46-59","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Absence of interleukin 22 affects the oral microbiota and the progression of induced periapical lesions in murine teeth"],"prefix":"10.1111","volume":"48","author":[{"given":"K. M. H.","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Department of Pediatric Clinics School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. A. B.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Pediatric Clinics School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"De Rossi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Clinics School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Y.","family":"Fukada","sequence":"additional","affiliation":[{"name":"Department of Physics and Chemistry School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Feres","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dentistry University of Guarulhos Guarulhos S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Nelson\u2010Filho","sequence":"additional","affiliation":[{"name":"Department of Pediatric Clinics School of Dentistry of Ribeir\u00e3o Preto University of S\u00e3o Paulo Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. A. 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