{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:19:19Z","timestamp":1784197159929,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T00:00:00Z","timestamp":1410480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Sciences","doi-asserted-by":"crossref","award":["23390433"],"award-info":[{"award-number":["23390433"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To examine the temporospatial expression of dentine matrix protein 1 (<jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1; a noncollagenous protein involved in mineralized tissue formation), osteopontin (another noncollagenous protein detected during reparative dentinogenesis) and nestin (a marker of differentiating\/differentiated odontoblasts), following direct pulp capping with calcium hydroxide in rat molars.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The maxillary first molars of 8\u2010week\u2010old Wistar rats had their pulps exposed and capped with calcium hydroxide. The pulp\u2010capped teeth were collected from 6\u00a0h to 14\u00a0days postoperatively and processed for immunohistochemistry for <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1, osteopontin and nestin. Cell proliferation was monitored using 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdU) labelling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The capped pulps initially exhibited superficial necrotic changes followed by the formation of new matrix and its mineralization. <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1 immunoreactivity was observed in the matrix beneath the necrotic layer from 6\u00a0h onwards and present in the outer portion of the newly formed mineralized matrix from 7\u00a0days onwards. Osteopontin displayed a similar expression pattern, although it occupied a narrower area than <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1 at 6 and 12\u00a0h. Nestin\u2010immunoreactive cells appeared beneath the <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1\u2010immunoreactive area at 1\u00a0day, were distributed beneath the newly formed matrix at 5\u00a0days and exhibited odontoblast\u2010like morphology by 14\u00a0days. BrdU\u2010positive cells significantly increased at 2 and 3\u00a0days (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) and then decreased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The deposition of <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1 at exposed pulp sites preceded the appearance of nestin\u2010immunoreactive cells, active cell proliferation and new matrix formation after pulp capping with calcium hydroxide in rat molars, suggesting that <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1 acts as a trigger of pulp repair. The colocalization of <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>1 and osteopontin suggests that these two proteins play complementary roles.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12351","type":"journal-article","created":{"date-parts":[[2014,7,30]],"date-time":"2014-07-30T07:54:27Z","timestamp":1406706867000},"page":"573-581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Temporospatial localization of dentine matrix protein 1 following direct pulp capping with calcium hydroxide in rat molars"],"prefix":"10.1111","volume":"48","author":[{"given":"Y.","family":"Shigetani","sequence":"first","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Yoshiba","sequence":"additional","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kuratate","sequence":"additional","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Takei","sequence":"additional","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Yoshiba","sequence":"additional","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Yamanaka","sequence":"additional","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ohshima","sequence":"additional","affiliation":[{"name":"Division of Anatomy and Cell Biology of the Hard Tissue Department of Tissue Regeneration and Reconstruction Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Okiji","sequence":"additional","affiliation":[{"name":"Division of Cariology, Operative Dentistry and Endodontics Department of Oral Health Science Niigata University Graduate School of Medical and Dental Sciences Niigata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64539-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302687"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2004.07.008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v018a08"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.02.001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1997.12.12.2040"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200390152061"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.4146"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(90)90093-P"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"12624","DOI":"10.1016\/S0021-9258(18)31434-0","article-title":"Characterization of a novel dentin matrix acidic phosphoprotein. 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