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After their histopathological categorization as periapical granulomas (<jats:italic>n<\/jats:italic>\u00a0=\u00a020), they were examined by immunohistochemistry using human LEPR monoclonal antibodies. LEPR <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was also determined by quantitative real\u2010time PCR (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010PCR), and the amount of LEPR protein was analysed by immunoblot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All granuloma samples expressed LEPR. Amongst inflammatory cells, only macrophages showed expression of LEPR. Western blot analysis revealed the presence in the samples of a protein with apparent molecular weight of ~120\u00a0kDa, corresponding to the estimated molecular weight of LEPR. The <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010PCR analysis demonstrated the expression of LEPR <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>, corresponding the size of the amplified fragment (338 bp), assessed by agarose gel electrophoresis, to that of LEPR <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Human periapical granulomas express <jats:styled-content style=\"fixed-case\">LEPR<\/jats:styled-content>. In periapical granulomas, only macrophages showed expression of <jats:styled-content style=\"fixed-case\">LEPR<\/jats:styled-content>. This finding suggests that leptin can play a role in inflammatory and immune periapical responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12356","type":"journal-article","created":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T15:21:00Z","timestamp":1406820060000},"page":"611-618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Expression and immunohistochemical localization of leptin receptor in human periapical granuloma"],"prefix":"10.1111","volume":"48","author":[{"given":"J.","family":"Mart\u00edn\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Department of Stomatology (Endodontics Section) School of Dentistry Virgen Macarena University Hospital University of Sevilla Sevilla Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Carmona\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena University Hospital University of Sevilla Sevilla Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"P\u00e9rez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena University Hospital University of Sevilla Sevilla Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"S\u00e1nchez\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena University Hospital University of Sevilla Sevilla Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"S\u00e1nchez\u2010Margalet","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Virgen Macarena University Hospital University of Sevilla Sevilla Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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