{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T10:42:39Z","timestamp":1784371359722,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,9,11]],"date-time":"2014-09-11T00:00:00Z","timestamp":1410393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate and compare the immunoexpression of tryptase in samples of periapical granulomas (<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s) and radicular cysts (<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s) correlating it with the type of lesion, localization, intensity of the inflammatory infiltrate and thickness of the cystic epithelial lining, in order to gain insight into the phlogistic role of these cells in the lesions studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Twenty\u2010five <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s and twenty\u2010five <jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s obtained from human teeth without endodontic treatment were submitted to morphological and immunohistochemical analysis using anti\u2010tryptase antibody. Mast cells were identified and counted in three regions: intra\u2010epithelial, central\/superficial and deep portions. The data were analysed using the <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ann\u2013<jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>hitney <jats:italic><jats:styled-content style=\"fixed-case\">U<\/jats:styled-content><\/jats:italic>\u2010test (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In comparison with <jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s exhibited higher immunoexpression of tryptase\u2010positive mast cells located in both central\/superficial and deep regions (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001 and <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001, respectively). When considering the total number of mast cells and disregarding the location, the number of tryptase\u2010positive mast cells increased gradually from <jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s to <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001). Lesions with inflammatory infiltrate grade <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> had greater number of tryptase\u2010positive mast cells located in both central\/superficial and deep regions than lesions with inflammatory infiltrates grade <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.045 and <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.025). When the location was ignored, the lesions with inflammatory infiltrate grade <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> also exhibited higher immunostaining of tryptase\u2010positive mast cells (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Tryptase\u2010positive mast cells were present in chronic periapical lesions in a larger number in periapical granulomas than in radicular cysts, in both central\/superficial and deep regions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12366","type":"journal-article","created":{"date-parts":[[2014,8,5]],"date-time":"2014-08-05T14:17:20Z","timestamp":1407248240000},"page":"729-735","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Immunoexpression of tryptase\u2010positive mast cells in periapical granulomas and radicular cysts"],"prefix":"10.1111","volume":"48","author":[{"given":"H.","family":"Costa Neto","sequence":"first","affiliation":[{"name":"Department of Dentistry Federal University of Rio Grande do Norte Natal RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. L. D. L.","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Department of Dentistry Federal University of Rio Grande do Norte Natal RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Gord\u00f3n\u2010N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"Department of Dentistry State University of Para\u00edba Araruna PB Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. de A.","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Dentistry Federal University of Rio Grande do Norte Natal RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. C.","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Dentistry Federal University of Rio Grande do Norte Natal RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.04.028"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2018"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2007.00509.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2007.00460.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(87)90292-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2009.00870.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-3400-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2012.02043.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2007.09.009"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"2310","DOI":"10.4049\/jimmunol.158.5.2310","article-title":"Human mast cells activate fibroblasts: tryptase is a fibrogenic factor stimulating collagen messenger ribonucleic acid synthesis and fibroblast chemotaxis","volume":"158","author":"Gruber BL","year":"1997","journal-title":"Journal of Immunology"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:22:2:107"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-1546.2004.00096.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.DON.0000134207.67360.FC"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.08.024"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.03.020"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.05.002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2000.00276.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2007.00595.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130401500604"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2012.01126.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2008.06.028"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.02.005"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(07)80157-9"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(03)00378-0"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.09.020"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1996.tb00282.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi972119z"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0904-2512.2004.00133.x"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiej.12366","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.12366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T16:37:11Z","timestamp":1696264631000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.12366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,11]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/iej.12366"],"URL":"https:\/\/doi.org\/10.1111\/iej.12366","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,11]]},"assertion":[{"value":"2014-02-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-08-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}