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No treatment was performed on the negative control. Immediately after bleaching, the cell viability, gene expression of inflammatory mediators and quantification of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> diffusion were evaluated. The <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> activity, <jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>\u20101 gene expression and mineralized nodule deposition (<jats:styled-content style=\"fixed-case\">MND<\/jats:styled-content>) were assessed at 7, 14 or 21\u00a0days post\u2010bleaching and analysed statistically with Mann\u2013Whitney <jats:italic>U<\/jats:italic>\u2010tests (\u03b1\u00a0=\u00a05%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> diffusion, proportional to treatment time, was observed in all bleached groups. Reductions of approximately 31%, 21% and 13% in cell viability were observed for the 45\u2010, 15\u2010 and 5\u2010min groups, respectively. This reduction was significant (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) for the 45\u2010 and 15\u2010min groups, which also presented significant (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) over\u2010expression of inflammatory mediators. The 45\u2010min group was associated with significant (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) reductions in <jats:styled-content style=\"fixed-case\">DMP<\/jats:styled-content>\u20101\/<jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> expression at all periods, relative to control. The <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> activity and <jats:styled-content style=\"fixed-case\">MND<\/jats:styled-content> were reduced only in initial periods. The 15\u2010min group had less intense reduction of all markers, with no difference to control at 21\u00a0days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The 17.5% H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> applied to tooth specimens for 5\u00a0min caused no alteration in the odontoblast\u2010like cells. When this gel was applied for 45 or 15\u00a0min, a slight cytotoxicity, associated with alterations in phenotypic markers, was observed. However, cells were able to recover their functions up to 21\u00a0days post\u2010bleaching.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12426","type":"journal-article","created":{"date-parts":[[2014,12,30]],"date-time":"2014-12-30T07:21:38Z","timestamp":1419924098000},"page":"26-36","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Indirect cytocompatibility of a low\u2010concentration hydrogen peroxide bleaching gel to odontoblast\u2010like cells"],"prefix":"10.1111","volume":"49","author":[{"given":"D. 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