{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T02:14:11Z","timestamp":1787364851100,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T00:00:00Z","timestamp":1444780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Guandong Natural Science Foundation","doi-asserted-by":"publisher","award":["S2013010014879"],"award-info":[{"award-number":["S2013010014879"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2016,10]]},"abstract":"Abstract<\/jats:title>\n \n Aim<\/jats:title>\n \n To investigate the\n in\u00a0vitro<\/jats:italic>\n effects of\n TNF<\/jats:styled-content>\n \u2010\u03b1 and\n IL<\/jats:styled-content>\n \u20101\u03b2 on the osteogenic\/dentinogenic differentiation of stem cells from the apical papilla (\n SCAP<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n SCAP<\/jats:styled-content>\n s treated with 10\u00a0ng\u00a0mL\n \u22121<\/jats:sup>\n TNF<\/jats:styled-content>\n \u2010\u03b1 and\/or 5\u00a0ng\u00a0mL\n \u22121<\/jats:sup>\n IL<\/jats:styled-content>\n \u20101\u03b2 were defined as the treatment groups, with untreated cells defined as the control group. Cell proliferation was measured using the\n MTT<\/jats:styled-content>\n assay and cell cycle analysis. Cells in all of the groups were cultured in the osteogenic\/dentinogenic differentiation medium and processed for analysis at days 3, 7 and 14. The Alizarin Red S assay, alkaline phosphatase staining and real\u2010time\n PCR<\/jats:styled-content>\n were used to evaluate the differentiation capacity. Differences between the treatment groups and the control groups were analysed statistically using one\u2010way\n anova<\/jats:sc>\n and Dunnett's post\u2010test.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Compared with the control group, the treated\n SCAP<\/jats:styled-content>\n s were associated with a significantly greater proliferation activity at days 3 and 5 but reduced proliferation at day 9 (\n P\u00a0<\u00a0<\/jats:italic>\n 0.05). Significantly lower calcium deposition and\n ALP<\/jats:styled-content>\n activity were observed in the treatment groups at day 14 (\n P\u00a0<\u00a0<\/jats:italic>\n 0.05), as well as reduced\n ALP<\/jats:styled-content>\n ,\n DSPP<\/jats:styled-content>\n and\n DMP<\/jats:styled-content>\n \u20101 expression levels (\n P\u00a0<\u00a0<\/jats:italic>\n 0.05); however, treated\n SCAP<\/jats:styled-content>\n s had significantly higher levels of\n DSPP<\/jats:styled-content>\n and\n DMP<\/jats:styled-content>\n \u20101 at day 7 (\n P\u00a0<\u00a0<\/jats:italic>\n 0.05). In the control group, the\n ALP<\/jats:styled-content>\n ,\n DSPP<\/jats:styled-content>\n and\n DMP<\/jats:styled-content>\n \u20101 expression levels increased significantly from day 3 to day 14 (\n P\u00a0<\u00a0<\/jats:italic>\n 0.05), but the treatment groups were not associated with increased expression from day 7 to day 14 (\n P\u00a0>\u00a0<\/jats:italic>\n 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The pro\u2010inflammatory cytokines\n TNF<\/jats:styled-content>\n \u2010\u03b1 and\n IL<\/jats:styled-content>\n \u20101\u03b2 inhibited mineralization and osteogenic\u2010\/dentinogenic\u2010related gene expression in\n SCAP<\/jats:styled-content>\n s\n in\u00a0vitro<\/jats:italic>\n after long\u2010term culturing. However, these cytokines induced cell proliferation and mineralization in short\u2010term culture probably as a protective response. These findings help to elucidate the impact of inflammation on tooth development.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/iej.12551","type":"journal-article","created":{"date-parts":[[2015,9,18]],"date-time":"2015-09-18T11:23:56Z","timestamp":1442575436000},"page":"950-959","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Long\u2010term exposure to pro\u2010inflammatory cytokines inhibits the osteogenic\/dentinogenic differentiation of stem cells from the apical papilla"],"prefix":"10.1111","volume":"49","author":[{"given":"C.","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Stomatology Guangzhou Women and Children's Medical Center Guangzhou Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Stomatology Guangzhou Women and Children's Medical Center 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