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Expression of Notch\u2010related genes was detected by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. A rat pulpitis model was established by mechanical injury and <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> plus mechanical injury was followed by the analysis of expression of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>otch2 by immunohistochemical staining. One\u2010way analysis of variance (<jats:sc>anova<\/jats:sc>) was performed to examine the effect of differing concentrations of <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> on cell proliferation, and least significant difference test was used for paired comparisons. For independent sample, <jats:italic>t<\/jats:italic>\u2010test was performed to compare the expression of Notch signalling genes between <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> group and control group <jats:italic>in\u00a0vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:italic>in\u00a0vitro<\/jats:italic> study revealed the proliferation of <jats:styled-content style=\"fixed-case\">MDPC<\/jats:styled-content>\u201023 cells on exposure to 10\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup> to 1\u00a0\u03bcg\u00a0mL<jats:sup>\u22121<\/jats:sup> <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>. Expression of Notch1 and Notch2 was significantly higher in the <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> group than that in the control group on day 1 and day 3 (<jats:italic>P\u00a0<\/jats:italic>\u02c2\u00a00.05). The levels of both Delta1 and Jagged1 were higher in the study group than in the control group on day 3 (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.019 and <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.034) and day 5 (<jats:italic>P\u00a0<\/jats:italic>\u02c2\u00a00.001 and <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.046), respectively. In addition, Hes1 levels were significantly higher in the study group than in the control group on day 5 (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.005). The <jats:italic>in\u00a0vivo<\/jats:italic> study demonstrated positive staining for Notch2, both in the mechanical injury (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>) group and in the <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> plus mechanical injury (<jats:styled-content style=\"fixed-case\">LMI<\/jats:styled-content>) group from day 3 to day 7, which showed very weak or absent staining on day 14, thereby demonstrating the dynamic nature of the change.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic> activation and dynamic expression of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>otch signalling in dental pulp cells after injury were found. Notch signalling activation by <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> stimulation or mechanical injury showed a similar pattern <jats:italic>in\u00a0vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12580","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T07:38:55Z","timestamp":1447745935000},"page":"1165-1174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Activation and dynamic expression of <scp>N<\/scp>otch signalling in dental pulp cells after injury <i>in\u00a0vitro<\/i> and <i>in\u00a0vivo<\/i>"],"prefix":"10.1111","volume":"49","author":[{"given":"L.","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Military Stomatology Department of Operative Dentistry &amp; Endodontics The Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. C.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry School of Stomatology The Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry School of Stomatology The Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry School of Stomatology The Fourth Military Medical University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. 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