{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T09:32:21Z","timestamp":1763544741676},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371134","31170894","81400504"],"award-info":[{"award-number":["81371134","31170894","81400504"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the effects of mammalian homologue of Drosophila diaphanous\u20101(<jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1) and Rho\u2010associated coiled\u2010coil\u2010containing protein kinase (<jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content>) on the migration and adhesion of dental pulp cells (<jats:styled-content style=\"fixed-case\">DPC<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Lysophosphatidic acid (<jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content>) was used to activate Rho signalling. <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 and <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> were inhibited by short interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and the specific inhibitor, Y\u201027632, respectively. The migration of <jats:styled-content style=\"fixed-case\">DPC<\/jats:styled-content>s was assessed using the transwell migration assay and scratch test. Formation of cytoskeleton and focal adhesions(FAs) was observed by confocal laser scanning microscopy. Cell adhesion and spreading assays were performed. Phosphorylation of focal adhesion kinase (<jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content>) and paxillin was detected by Western blotting, and the bands were analysed using Adobe Photoshop <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>5 software. All experiments were performed at least three times, and data were analysed with one\u2010way <jats:sc>anova<\/jats:sc> and a <jats:italic>post hoc<\/jats:italic> test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content>\u2010triggered activation of Rho and inhibition of <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> significantly increased the cell migration rate. Cell migration was inhibited by silencing <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1. <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 silencing and <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> inhibition suppressed the <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content>\u2010induced formation of the cytoskeleton, FA and phosphorylation of <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content> and paxillin. Inhibition of <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 facilitated early cell adhesion and spreading; by contrast, the combined inhibition of <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 neutralized these effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 promoted RhoA\u2010induced migration of <jats:styled-content style=\"fixed-case\">DPC<\/jats:styled-content>s, but <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> had an opposite effect. Both <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 and <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> participated in cytoskeleton formation and adhesion of <jats:styled-content style=\"fixed-case\">DPC<\/jats:styled-content>s. The interactions between <jats:styled-content style=\"fixed-case\">mD<\/jats:styled-content>ia1 and <jats:styled-content style=\"fixed-case\">ROCK<\/jats:styled-content> might influence dental pulp repair by determining the migration and adhesion of DPCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12587","type":"journal-article","created":{"date-parts":[[2015,11,26]],"date-time":"2015-11-26T15:26:07Z","timestamp":1448551567000},"page":"15-23","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Interaction between <scp>mD<\/scp>ia1 and <scp>ROCK<\/scp> in Rho\u2010induced migration and adhesion of human dental pulp cells"],"prefix":"10.1111","volume":"50","author":[{"given":"L.","family":"Cheng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Y.","family":"Qian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Y.","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Y.","family":"Shao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"},{"name":"College of Life Sciences Sichuan University Chengdu Sichuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu Sichuan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology Chengdu 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