{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:50Z","timestamp":1787014310342,"version":"build-2736575974"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T00:00:00Z","timestamp":1452038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Irish Endodontic Society","award":["2008\/1"],"award-info":[{"award-number":["2008\/1"]}]},{"name":"European Society of Endodontology","award":["2012"],"award-info":[{"award-number":["2012"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2017,1]]},"abstract":"Abstract<\/jats:title>\n \n Aim<\/jats:title>\n \n To characterize dentine matrix component (\n DMC<\/jats:styled-content>\n ) release and smear layer removal by histone deacetylase inhibitors (\n HDAC<\/jats:styled-content>\n is).\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n DMC<\/jats:styled-content>\n s were extracted from powdered human dentine over 14\u00a0days using three\n HDAC<\/jats:styled-content>\n is, valproic acid (\n VPA<\/jats:styled-content>\n ), trichostatin A (\n TSA<\/jats:styled-content>\n ) and suberoylanilide hydroxamic acid (\n SAHA<\/jats:styled-content>\n ) and compared with a control extractant, 10% (w\/v)\n EDTA<\/jats:styled-content>\n . Protein compositions of the resultant extracts were analysed by 1D\u2010polyacrylamide gel electrophoresis (1D\u2010\n PAGE<\/jats:styled-content>\n ),\n TGF<\/jats:styled-content>\n \u2010\u03b2\u20101 and\n MMP<\/jats:styled-content>\n \u20109\n ELISA<\/jats:styled-content>\n s and a high\u2010throughput growth factor antibody array. Dentine discs with a standardized smear layer were prepared from human molars and treated with\n EDTA<\/jats:styled-content>\n (17% w\/v), polyacrylic acid (\n PA<\/jats:styled-content>\n ) (20% v\/v) and the experimental\n HDAC<\/jats:styled-content>\n is prior to analysis by scanning electron microscopy. Parametric\n ELISA<\/jats:styled-content>\n data were analysed using one\u2010way\n anova<\/jats:sc>\n and Tukey's\n post hoc<\/jats:italic>\n test, whilst nonparametric smear layer data were analysed by Kruskal\u2013Wallis test and Mann\u2013Whitney\n U<\/jats:italic>\n \u2010test (\n P<\/jats:italic>\n \u00a0<\u00a00.05).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n HDAC<\/jats:styled-content>\n is did not remove smear layer in the presence or absence of\n PA<\/jats:styled-content>\n pre\u2010treatment (\n P<\/jats:italic>\n \u00a0\u2265\u00a00.478). 1D\u2010\n PAGE<\/jats:styled-content>\n analysis demonstrated different protein profiles for\n EDTA<\/jats:styled-content>\n and\n HDAC<\/jats:styled-content>\n i extracts. All\n HDAC<\/jats:styled-content>\n i solutions released\n TGF<\/jats:styled-content>\n \u2010\u03b2\u20101 although less effectively than\n EDTA<\/jats:styled-content>\n (\n P<\/jats:italic>\n \u00a0<\u00a00.001), whilst\n MMP<\/jats:styled-content>\n \u20109 was extracted in significantly higher concentration by\n EDTA<\/jats:styled-content>\n and\n VPA<\/jats:styled-content>\n compared with\n TSA<\/jats:styled-content>\n (\n P<\/jats:italic>\n \u00a0<\u00a00.012). Antibody array analysis demonstrated the ability of\n HDAC<\/jats:styled-content>\n is to extract a complex cocktail of established\/novel growth factors from dentine, albeit significantly less efficiently than\n EDTA<\/jats:styled-content>\n for certain cytokines (\n TGF<\/jats:styled-content>\n \u2010\u03b2\u20101,\n PDGF<\/jats:styled-content>\n \u2010\n AA<\/jats:styled-content>\n ,\n VEGF<\/jats:styled-content>\n \u2010A) and significantly more effectively for others (\n GDF<\/jats:styled-content>\n \u201015,\n IGF<\/jats:styled-content>\n \u20101,\n EGRF<\/jats:styled-content>\n \u20101,\n NGFR<\/jats:styled-content>\n ,\n BDNF<\/jats:styled-content>\n ,\n SCF<\/jats:styled-content>\n \u2010R).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n HDAC<\/jats:styled-content>\n i release a range of bioactive\n DMC<\/jats:styled-content>\n s that could promote dentine repair processes\n in\u00a0vivo<\/jats:italic>\n ; however, they are ineffective at removing smear layer.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/iej.12588","type":"journal-article","created":{"date-parts":[[2015,11,26]],"date-time":"2015-11-26T08:36:52Z","timestamp":1448527012000},"page":"24-38","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Release of bio\u2010active dentine extracellular matrix components by histone deacetylase inhibitors (\n HDAC<\/scp>\n i)"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8690-2379","authenticated-orcid":false,"given":"H. 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