{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T17:46:09Z","timestamp":1759340769957,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T00:00:00Z","timestamp":1459814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A_140868","CR32I3_152809"],"award-info":[{"award-number":["31003A_140868","CR32I3_152809"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To analyse the effect of systemic application of N\u2010methyl pyrrolidone (<jats:styled-content style=\"fixed-case\">NMP<\/jats:styled-content>) on the pulp\u2013dentine complex and on the jawbone of ovariectomized rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Female Sprague Dawley rats were randomly divided into a Sham\u2010operated group (Sham <jats:italic>n<\/jats:italic>\u00a0=\u00a06) and an oestrogen depletion by ovariectomy (<jats:styled-content style=\"fixed-case\">OVX <\/jats:styled-content><jats:italic>n<\/jats:italic>\u00a0=\u00a012) group. In 6 of the ovariectomized animals, N\u2010methyl pyrrolidone (<jats:styled-content style=\"fixed-case\">NMP<\/jats:styled-content>) in phosphate\u2010buffered saline (<jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>) was administered systemically weekly by intraperitoneal injection (i.p.); the other 6 were injected with <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content> (Veh). After 15\u00a0weeks of injections, the jaw bones were collected and pulps extracted from the incisors teeth. Histology was used to determine pre\u2010dentine thickness in teeth and radiography to determine alveolar bone mass. Immunohistological staining and <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> were performed to verify the presence and localization of the odontoblast\u2010specific dentine sialoprotein and to quantify its expression in the dentine\u2013pulp complex. Mandibular cortical width and mandibular height were evaluated by means of X\u2010ray analysis. Statistical analysis was performed with analysis of variance (<jats:sc>anova<\/jats:sc>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both pre\u2010dentine (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.029) and alveolar bone structures (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.049) were significantly reduced due to oestrogen deficiency in <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> Veh and <jats:styled-content style=\"fixed-case\">OVX. NMP<\/jats:styled-content> treatment normalized these parameters to the Sham level. <jats:styled-content style=\"fixed-case\">DSPP<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">OVX NMP<\/jats:styled-content> animals was significantly higher (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.046) than in <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> Veh. X\u2010ray analysis confirmed that ovariectomy significantly reduced the mandibular cortical width in the <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> Veh group compared to the Sham Veh and <jats:styled-content style=\"fixed-case\">OVX NMP<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.020).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>N\u2010methyl pyrrolidone (<jats:styled-content style=\"fixed-case\">NMP<\/jats:styled-content>) had a remarkable anti\u2010osteoporotic ability preserving activity in the pulp\u2013dentine complex and preventing jawbone loss. These effects make <jats:styled-content style=\"fixed-case\">NMP<\/jats:styled-content> a promising candidate for the preservation of the activity of the pulp\u2013dentine complex and jawbone thickness in post\u2010menopausal females.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12622","type":"journal-article","created":{"date-parts":[[2016,2,26]],"date-time":"2016-02-26T03:16:35Z","timestamp":1456456595000},"page":"271-280","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Influence of N\u2010methyl pyrrolidone on the activity of the pulp\u2013dentine complex and bone integrity during osteoporosis"],"prefix":"10.1111","volume":"50","author":[{"given":"B.","family":"Gjoksi","sequence":"first","affiliation":[{"name":"Oral Biotechnology &amp; Bioengineering Center of Dental Medicine Cranio\u2010Maxillofacial and Oral Surgery University of Zurich Zurich Switzerland"},{"name":"Cartilage engineering + Regeneration Laboratory ETH Zurich Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ruangsawasdi","sequence":"additional","affiliation":[{"name":"Oral Biotechnology &amp; Bioengineering Center of Dental Medicine Cranio\u2010Maxillofacial and Oral Surgery University of Zurich Zurich Switzerland"},{"name":"Zurich Center for Integrative Human Physiology (ZIHP) University of Zurich Zurich Switzerland"},{"name":"Department Pharmacology Faculty of Dentistry Mahidol University Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Ghayor","sequence":"additional","affiliation":[{"name":"Oral Biotechnology &amp; Bioengineering Center of Dental Medicine Cranio\u2010Maxillofacial and Oral Surgery University of Zurich Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Siegenthaler","sequence":"additional","affiliation":[{"name":"Oral Biotechnology &amp; Bioengineering Center of Dental Medicine Cranio\u2010Maxillofacial and Oral Surgery University of Zurich Zurich Switzerland"},{"name":"Zurich Center for Integrative Human Physiology (ZIHP) University of Zurich Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Zenobi\u2010Wong","sequence":"additional","affiliation":[{"name":"Cartilage engineering + Regeneration Laboratory ETH Zurich Zurich Switzerland"},{"name":"CABMM Center for Applied Biotechnology and Molecular Medicine University of Zurich Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1670-2296","authenticated-orcid":false,"given":"F. 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